Anti-MAP3K9 antibody (ab154506)
Key features and details
- Rabbit polyclonal to MAP3K9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP3K9 antibody
See all MAP3K9 primary antibodies -
Description
Rabbit polyclonal to MAP3K9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to a region within amino acids 198-433 of Human MAP3K9 (P80192).
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Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates. A431 cells.
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General notes
Keep as concentrated solution.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Activates the JUN N-terminal pathway. -
Tissue specificity
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. - Information by UniProt
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Database links
- Entrez Gene: 4293 Human
- Entrez Gene: 338372 Mouse
- Omim: 600136 Human
- SwissProt: P80192 Human
- SwissProt: Q3U1V8 Mouse
- Unigene: 445496 Human
- Unigene: 593542 Human
- Unigene: 35284 Mouse
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Alternative names
- M3K9_HUMAN antibody
- Map3k9 antibody
- MEKK9 antibody
see all
Images
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Western blot - Anti-MAP3K9 antibody (ab154506)Anti-MAP3K9 antibody (ab154506) at 1/500 dilution + A431 whole cell
lysate at 30 µg
Predicted band size: 122 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling MAP3K9 with ab154506 at 1/200 dilution. Image at the bottom merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154506 has not yet been referenced specifically in any publications.