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Cell Biology Apoptosis Intracellular Kinases
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Anti-MAP3K9 antibody (ab154506)

  • Datasheet
  • SDS
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Western blot - Anti-MAP3K9 antibody (ab154506)
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)

Key features and details

  • Rabbit polyclonal to MAP3K9
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human MAP3K9 protein (ab125554)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MAP3K9 antibody
    See all MAP3K9 primary antibodies
  • Description

    Rabbit polyclonal to MAP3K9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 198-433 of Human MAP3K9 (P80192).

  • Positive control

    • A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates. A431 cells.
  • General notes

    Keep as concentrated solution.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human MAP3K9 protein (ab125554)
  • Related Products

    • Recombinant human MAP3K9 protein (ab125554)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Activates the JUN N-terminal pathway.
  • Tissue specificity

    Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Target information above from: UniProt accession P80192 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4293 Human
    • Entrez Gene: 338372 Mouse
    • Omim: 600136 Human
    • SwissProt: P80192 Human
    • SwissProt: Q3U1V8 Mouse
    • Unigene: 445496 Human
    • Unigene: 593542 Human
    • Unigene: 35284 Mouse
    • Alternative names

      • M3K9_HUMAN antibody
      • Map3k9 antibody
      • MEKK9 antibody
      • Mitogen activated protein kinase kinase kinase 9 antibody
      • Mitogen-activated protein kinase kinase kinase 9 antibody
      • Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody
      • Mixed lineage kinase 1 antibody
      • MLK1 antibody
      • PRKE1 antibody
      see all

    Images

    • Western blot - Anti-MAP3K9 antibody (ab154506)
      Western blot - Anti-MAP3K9 antibody (ab154506)
      Anti-MAP3K9 antibody (ab154506) at 1/500 dilution + A431 whole cell
      lysate at 30 µg

      Predicted band size: 122 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)
      Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)
      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling MAP3K9 with ab154506 at 1/200 dilution. Image at the bottom merged with DNA probe.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154506? Please let us know so that we can cite the reference in this datasheet.

    ab154506 has not yet been referenced specifically in any publications.

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