Anti-MAP3K9 antibody (ab154506)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-MAP3K9 antibody
See all MAP3K9 primary antibodies -
DescriptionRabbit polyclonal to MAP3K9
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to a region within amino acids 198-433 of Human MAP3K9 (P80192).
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Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates. A431 cells.
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General notesKeep as concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 122 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionActivates the JUN N-terminal pathway.
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Tissue specificityExpressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
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Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. - Information by UniProt
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Database links
- Entrez Gene: 4293 Human
- Entrez Gene: 338372 Mouse
- Omim: 600136 Human
- SwissProt: P80192 Human
- SwissProt: Q3U1V8 Mouse
- Unigene: 445496 Human
- Unigene: 593542 Human
- Unigene: 35284 Mouse
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Alternative names
- M3K9_HUMAN antibody
- Map3k9 antibody
- MEKK9 antibody
see all
Images
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Western blot - Anti-MAP3K9 antibody (ab154506)Anti-MAP3K9 antibody (ab154506) at 1/500 dilution + A431 whole cell
lysate at 30 µg
Predicted band size: 122 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling MAP3K9 with ab154506 at 1/200 dilution. Image at the bottom merged with DNA probe.
References
ab154506 has not yet been referenced specifically in any publications.
