Overview

  • Product name
    Anti-MAP3K9 antibody
    See all MAP3K9 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 198-433 of Human MAP3K9 (P80192).

  • Positive control
    • A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates. A431 cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 122 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Activates the JUN N-terminal pathway.
  • Tissue specificity
    Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Information by UniProt
  • Database links
    • Alternative names
      • M3K9_HUMAN antibody
      • Map3k9 antibody
      • MEKK9 antibody
      • Mitogen activated protein kinase kinase kinase 9 antibody
      • Mitogen-activated protein kinase kinase kinase 9 antibody
      • Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody
      • Mixed lineage kinase 1 antibody
      • MLK1 antibody
      • PRKE1 antibody
      see all

    Images

    • Western blot - Anti-MAP3K9 antibody (ab154506)
      Western blot - Anti-MAP3K9 antibody (ab154506)
      Anti-MAP3K9 antibody (ab154506) at 1/500 dilution + A431 whole cell
      lysate at 30 µg

      Predicted band size: 122 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)
      Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (ab154506)
      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling MAP3K9 with ab154506 at 1/200 dilution. Image at the bottom merged with DNA probe.

    References

    ab154506 has not yet been referenced specifically in any publications.

    Publishing research using ab154506? Please let us know so that we can cite the reference in this datasheet.

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