Anti-MAP3K9 antibody (ab228752)
Key features and details
- Rabbit polyclonal to MAP3K9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP3K9 antibody
See all MAP3K9 primary antibodies -
Description
Rabbit polyclonal to MAP3K9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human MAP3K9 (internal sequence). The exact sequence is proprietary.
Database link: P80192 -
Positive control
- WB: Jurkat, Raji and NCI-H1299 whole cell extracts. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Activates the JUN N-terminal pathway. -
Tissue specificity
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. - Information by UniProt
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Database links
- Entrez Gene: 538340 Cow
- Entrez Gene: 4293 Human
- Entrez Gene: 338372 Mouse
- Entrez Gene: 500690 Rat
- Omim: 600136 Human
- SwissProt: P80192 Human
- SwissProt: Q3U1V8 Mouse
- Unigene: 445496 Human
see all -
Alternative names
- M3K9_HUMAN antibody
- Map3k9 antibody
- MEKK9 antibody
see all
Images
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All lanes : Anti-MAP3K9 antibody (ab228752) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for MAP3K9 (green) using ab228752 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab228752 has been referenced in 4 publications.
- Ren Q et al. MicroRNA-361-5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9. Exp Ther Med 21:574 (2021). PubMed: 33850546
- Fan YC et al. Investigation of Anti-Tumor Effects of an MLK1 Inhibitor in Prostate and Pancreatic Cancers. Biology (Basel) 10:N/A (2021). PubMed: 34439974
- Jun L et al. Circ_SIPA1L1 Promotes Osteosarcoma Progression Via miR-379-5p/MAP3K9 Axis. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32897735
- Abdeyrim A et al. miR-490-5p regulates the proliferation, migration, invasion and epithelial-mesenchymal transition of pharyngolaryngeal cancer cells by targeting mitogen-activated protein kinase kinasekinase 9. Int J Mol Med 44:240-252 (2019). PubMed: 31115491