Product nameAnti-MAP3K9 antibody
See all MAP3K9 primary antibodies
DescriptionRabbit polyclonal to MAP3K9
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment within Human MAP3K9 (internal sequence). The exact sequence is proprietary.
Database link: P80192
- WB: Jurkat, Raji and NCI-H1299 whole cell extracts. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 122 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionActivates the JUN N-terminal pathway.
Tissue specificityExpressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
- Information by UniProt
- M3K9_HUMAN antibody
- Map3k9 antibody
- MEKK9 antibody
All lanes : Anti-MAP3K9 antibody (ab228752) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for MAP3K9 (green) using ab228752 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab228752 has not yet been referenced specifically in any publications.