Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP3K9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human MAP3K9 (internal sequence). The exact sequence is proprietary.
    Database link: P80192

  • Positive control

    • WB: Jurkat, Raji and NCI-H1299 whole cell extracts. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 122 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Activates the JUN N-terminal pathway.
  • Tissue specificity

    Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Information by UniProt
  • Database links

  • Alternative names

    • M3K9_HUMAN antibody
    • Map3k9 antibody
    • MEKK9 antibody
    • Mitogen activated protein kinase kinase kinase 9 antibody
    • Mitogen-activated protein kinase kinase kinase 9 antibody
    • Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody
    • Mixed lineage kinase 1 antibody
    • MLK1 antibody
    • PRKE1 antibody
    see all

Images

  • All lanes : Anti-MAP3K9 antibody (ab228752) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 122 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for MAP3K9 (green) using ab228752 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228752 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228752.
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