Key features and details
- Rabbit polyclonal to MAP4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP4 antibody
See all MAP4 primary antibodies
DescriptionRabbit polyclonal to MAP4
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: U-251 MG sp cell lysate; Human liver and tonsil lysates. IHC-P: Human heart muscle, skeletal muscle, and kidney tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 121 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNon-neuronal microtubule-associated protein. Promotes microtubule assembly.
Sequence similaritiesContains 4 Tau/MAP repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DKFZp779A1753 antibody
- MAP-4 antibody
- MAP4 antibody
Paraffin-embedded human kidney stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human soft tissue tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
All lanes : Anti-MAP4 antibody (ab224543) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 121 kDa
Paraffin-embedded human heart muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MAP4 (green) using ab224543 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224543 has not yet been referenced specifically in any publications.