Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MAP4 aa 300-350. The exact sequence is proprietary. NP_002366.2
    Database link: P27816

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 121 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Non-neuronal microtubule-associated protein. Promotes microtubule assembly.
  • Sequence similarities

    Contains 4 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp779A1753 antibody
    • MAP-4 antibody
    • MAP4 antibody
    • MAP4_HUMAN antibody
    • MGC8617 antibody
    • Microtubule associated protein 4 antibody
    • Microtubule-associated protein 4 antibody
    • OTTHUMP00000210723 antibody
    • OTTHUMP00000210725 antibody
    • OTTHUMP00000210727 antibody
    • OTTHUMP00000210730 antibody
    see all

Images

  • All lanes : Anti-MAP4 antibody (ab245577) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 121 kDa


    Exposure time: 1 second
  • MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245577 used for IP at 3 µg/mg lysate. For WB 0.01 µg/ml.

    Lane 1: ab245577 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 1 second.

References

ab245577 has not yet been referenced specifically in any publications.

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