Product nameAnti-MAP4 antibody
See all MAP4 primary antibodies
DescriptionRabbit polyclonal to MAP4
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human MAP4 aa 1102-1152. The exact sequence is proprietary. NP_002366.2
Database link: P27816
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245578 was affinity purified using an epitope specific to MAP4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 121 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNon-neuronal microtubule-associated protein. Promotes microtubule assembly.
Sequence similaritiesContains 4 Tau/MAP repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DKFZp779A1753 antibody
- MAP-4 antibody
- MAP4 antibody
All lanes : Anti-MAP4 antibody (ab245578) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 121 kDa
Exposure time: 30 seconds
MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245578 used for IP at 3 µg/mg lysate. For WB 0.01 µg/ml.
Lane 1: ab245578 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for MAP4 with ab245578 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
ab245578 has not yet been referenced specifically in any publications.