Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MAP4 aa 1102-1152. The exact sequence is proprietary. NP_002366.2
    Database link: P27816

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 121 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-MAP4 antibody (ab245578) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 121 kDa


    Exposure time: 30 seconds
  • MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245578 used for IP at 3 µg/mg lysate. For WB 0.01 µg/ml.

    Lane 1: ab245578 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 30 seconds.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for MAP4 with ab245578 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab245578 has not yet been referenced specifically in any publications.

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