Anti-MAP4K1/HPK1 antibody (ab129319)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (ab129319)
  • Western blot - Anti-MAP4K1/HPK1 antibody (ab129319)

Key features and details

  • Goat polyclonal to MAP4K1/HPK1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MAP4K1/HPK1 antibody
    See all MAP4K1/HPK1 primary antibodies

  • Description

    Goat polyclonal to MAP4K1/HPK1

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human MAP4K1/HPK1 aa 2-14 (N terminal) (Cysteine residue). (NP_009112.1)
    Sequence:

    DVVDPDIFNRDPR-C


    Database link: NP_001036065.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Thymus tissue; Jurkat cell lysate.

  • General notes

     This product was previously labelled as MAP4K1

     

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Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 91 kDa.
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 91 kDa.
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.

  • Tissue specificity

    Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • Hematopoietic progenitor kinase 1 antibody
      • Hematopoietic progenitor kinase antibody
      • HPK1 antibody
      • Human Hematopoietic Progenitor Kinase antibody
      • M4K1_HUMAN antibody
      • Map4k1 antibody
      • MAPK/ERK kinase kinase kinase 1 antibody
      • MEK kinase kinase 1 antibody
      • MEKKK 1 antibody
      • Mitogen activated protein kinase kinase kinase kinase 1 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (ab129319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (ab129319)

      ab129319 at 3.75 µg/ml staining MAP4K1/HPK1 in Formalin-Fixed, Paraffin-Embedded Human thymus tissue by Immunohistochemistry.

    • Western blot - Anti-MAP4K1/HPK1 antibody (ab129319)
      Western blot - Anti-MAP4K1/HPK1 antibody (ab129319)
      Anti-MAP4K1/HPK1 antibody (ab129319) at 0.5 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 91 kDa

    References (0)

    Publishing research using ab129319? Please let us know so that we can cite the reference in this datasheet.

    ab129319 has not yet been referenced specifically in any publications.

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