Anti-MAP4K1/HPK1 antibody (ab129319)
Key features and details
- Goat polyclonal to MAP4K1/HPK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4K1/HPK1 antibody
See all MAP4K1/HPK1 primary antibodies -
Description
Goat polyclonal to MAP4K1/HPK1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig, a wide range of other species -
Immunogen
Synthetic peptide corresponding to Human MAP4K1/HPK1 aa 2-14 (N terminal) (Cysteine residue). (NP_009112.1)
Sequence:DVVDPDIFNRDPR-C
Database link: NP_001036065.1 -
Positive control
- Human Thymus tissue; Jurkat cell lysate.
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General notes
This product was previously labelled as MAP4K1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 91 kDa.
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IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 91 kDa. |
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. -
Tissue specificity
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 522002 Cow
- Entrez Gene: 484527 Dog
- Entrez Gene: 11184 Human
- Entrez Gene: 100516374 Pig
- Omim: 601983 Human
- SwissProt: Q92918 Human
- Unigene: 95424 Human
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Alternative names
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (ab129319)
ab129319 at 3.75 µg/ml staining MAP4K1/HPK1 in Formalin-Fixed, Paraffin-Embedded Human thymus tissue by Immunohistochemistry.
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Anti-MAP4K1/HPK1 antibody (ab129319) at 0.5 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129319 has not yet been referenced specifically in any publications.