Product nameAnti-MAP4K1/HPK1 antibody [EP630Y]
See all MAP4K1/HPK1 primary antibodies
DescriptionRabbit monoclonal [EP630Y] to MAP4K1/HPK1
Specificityab33910 recognises hematopoietic progenitor kinase 1 (HPK1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human MAP4K1/HPK1 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MAP4K1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.09% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 97 kDa.|
FunctionMay play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
Tissue specificityExpressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
- Information by UniProt
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
All lanes : Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 97 kDa
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
This product has been referenced in:
- Lim S et al. Identification of the kinase STK25 as an upstream activator of LATS signaling. Nat Commun 10:1547 (2019). Read more (PubMed: 30948712) »
- Li Y et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med 38:803-11 (2016). Human . Read more (PubMed: 27430936) »