Key features and details
- Rabbit monoclonal [EP630Y] to MAP4K1/HPK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP4K1/HPK1 antibody [EP630Y]
See all MAP4K1/HPK1 primary antibodies
DescriptionRabbit monoclonal [EP630Y] to MAP4K1/HPK1
Specificityab33910 recognises hematopoietic progenitor kinase 1 (HPK1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human MAP4K1/HPK1 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MAP4K1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.09% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 97 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMay play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
Tissue specificityExpressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
- Information by UniProt
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
All lanes : Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 97 kDa
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab33910 has been referenced in 5 publications.
- Lim S et al. Identification of the kinase STK25 as an upstream activator of LATS signaling. Nat Commun 10:1547 (2019). PubMed: 30948712
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Chen Y et al. Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes. Cell Death Differ 26:1346-1364 (2019). PubMed: 30341421
- Li Y et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med 38:803-11 (2016). Human . PubMed: 27430936
- Palmer RD et al. Pediatric malignant germ cell tumors show characteristic transcriptome profiles. Cancer Res 68:4239-47 (2008). IHC-P ; Human . PubMed: 18519683