Anti-MAP4K4/NIK antibody (ab155583)
Key features and details
- Rabbit polyclonal to MAP4K4/NIK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-MAP4K4/NIK antibody
See all MAP4K4/NIK primary antibodies -
Description
Rabbit polyclonal to MAP4K4/NIK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MAP4K4/NIK aa 383-437.
-
Positive control
- 293T, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SNU1 xenograft tissue.
-
General notes
Keep as a concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 142 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 142 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. -
Tissue specificity
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 9448 Human
- Omim: 604666 Human
- SwissProt: O95819 Human
- Unigene: 431550 Human
-
Alternative names
- FLH21957 antibody
- FLJ10410 antibody
- FLJ20373 antibody
see all
Images
-
Anti-MAP4K4/NIK antibody (ab155583) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 142 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human SNU1 xenograft tissue labeling MAP4K4/NIK with ab155583 at 1/250 dilution.
-
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K4/NIK with ab155583 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab155583 has been referenced in 8 publications.
- Zhang J et al. Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer. Genes (Basel) 13:N/A (2022). PubMed: 36292671
- Chen S et al. Silencing CDC25A inhibits the proliferation of liver cancer cells by downregulating IL-6 in vitro and in vivo. Int J Mol Med 45:743-752 (2020). PubMed: 31922225
- Lemberger M et al. MicroRNA profiling of pancreatic ductal adenocarcinoma (PDAC) reveals signature expression related to lymph node metastasis. Oncotarget 10:2644-2656 (2019). PubMed: 31080555
- Dong XZ et al. Sanguinarine inhibits the proliferation of BGC-823 gastric cancer cells via regulating miR-96-5p/miR-29c-3p and the MAPK/JNK signaling pathway. J Nat Med 73:777-788 (2019). PubMed: 31243669
- Strang BL et al. Identification of lead anti-human cytomegalovirus compounds targeting MAP4K4 via machine learning analysis of kinase inhibitor screening data. PLoS One 13:e0201321 (2018). PubMed: 30048526
- Wang F et al. Chemotherapy-induced miR-141/MAP4K4 signaling suppresses progression of colorectal cancer. Biosci Rep 38:N/A (2018). PubMed: 30429233
- Liu YF et al. Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Mol Med Rep 13:41-8 (2016). PubMed: 26549737
- Feng L et al. Role of microRNA-141 in colorectal cancer with lymph node metastasis. Exp Ther Med 12:3405-3410 (2016). WB ; Human . PubMed: 27882171