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Rabbit polyclonal to MAP4K4
Synthetic peptide, corresponding to a region within amino acids 383-437 of Human MAP4K4.
293T, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SNU1 xenograft tissue.
Keep as a concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 142 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain. Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-MAP4K4 antibody (ab155583)
Anti-MAP4K4 antibody (ab155583) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 142 kDa 5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4 antibody (ab155583)
Immunohistochemical analysis of paraffin-embedded Human SNU1 xenograft tissue labeling MAP4K4 with ab155583 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MAP4K4 antibody (ab155583)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K4 with ab155583 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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