Anti-MAP4K4/NIK antibody (ab69885)
Key features and details
- Rabbit polyclonal to MAP4K4/NIK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4K4/NIK antibody
See all MAP4K4/NIK primary antibodies -
Description
Rabbit polyclonal to MAP4K4/NIK -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human MAP4K4/NIK (internal sequence).
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Positive control
- A549 cells. Extracts from COLO and HUVEC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 142 kDa (predicted molecular weight: 142 kDa).
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 142 kDa (predicted molecular weight: 142 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. -
Tissue specificity
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9448 Human
- Entrez Gene: 26921 Mouse
- Omim: 604666 Human
- SwissProt: O95819 Human
- SwissProt: P97820 Mouse
- Unigene: 431550 Human
- Unigene: 485049 Mouse
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Alternative names
- FLH21957 antibody
- FLJ10410 antibody
- FLJ20373 antibody
see all
Images
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All lanes : Anti-MAP4K4/NIK antibody (ab69885) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from HUVEC cells
Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 142 kDa
Observed band size: 142 kDa -
Immunofluorescence analysis of A549 cells using ab69885 at 1/500 dilution, in the presence or absence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab69885 has not yet been referenced specifically in any publications.