• Product name
  • Description
    Rabbit polyclonal to MAP4K4/NIK
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP4K4/NIK aa 383-437.

  • Positive control
    • 293T, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SNU1 xenograft tissue.
  • General notes
    Keep as a concentrated solution.

     This product was previously labelled as MAP4K4



  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab155583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 142 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.


  • Function
    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
  • Tissue specificity
    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • FLH21957 antibody
    • FLJ10410 antibody
    • FLJ20373 antibody
    • FLJ90111 antibody
    • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
    • HGK antibody
    • HPK/GCK like kinase antibody
    • HPK/GCK like kinase HGK antibody
    • HPK/GCK-like kinase HGK antibody
    • KIAA0687 antibody
    • M4K4_HUMAN antibody
    • MAP4K4 antibody
    • MAPK/ERK kinase kinase kinase 4 antibody
    • MEK kinase kinase 4 antibody
    • MEKKK 4 antibody
    • MEKKK4 antibody
    • Mitogen activated protein kinase kinase kinase kinase 4 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
    • Nck interacting kinase antibody
    • Nck-interacting kinase antibody
    • NIK antibody
    see all


  • Anti-MAP4K4/NIK antibody (ab155583) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 142 kDa

    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SNU1 xenograft tissue labeling MAP4K4/NIK with ab155583 at 1/250 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K4/NIK with ab155583 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.


This product has been referenced in:
  • Strang BL  et al. Identification of lead anti-human cytomegalovirus compounds targeting MAP4K4 via machine learning analysis of kinase inhibitor screening data. PLoS One 13:e0201321 (2018). Read more (PubMed: 30048526) »
  • Liu YF  et al. Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Mol Med Rep 13:41-8 (2016). Read more (PubMed: 26549737) »
See all 3 Publications for this product

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