Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP4K4/NIK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP4K4/NIK aa 383-437.

  • Positive control

    • 293T, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SNU1 xenograft tissue.
  • General notes

    Keep as a concentrated solution.

     This product was previously labelled as MAP4K4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 142 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
  • Tissue specificity

    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLH21957 antibody
    • FLJ10410 antibody
    • FLJ20373 antibody
    • FLJ90111 antibody
    • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
    • HGK antibody
    • HPK/GCK like kinase antibody
    • HPK/GCK like kinase HGK antibody
    • HPK/GCK-like kinase HGK antibody
    • KIAA0687 antibody
    • M4K4_HUMAN antibody
    • MAP4K4 antibody
    • MAPK/ERK kinase kinase kinase 4 antibody
    • MEK kinase kinase 4 antibody
    • MEKKK 4 antibody
    • MEKKK4 antibody
    • Mitogen activated protein kinase kinase kinase kinase 4 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
    • Nck interacting kinase antibody
    • Nck-interacting kinase antibody
    • NIK antibody
    see all

Images

  • Anti-MAP4K4/NIK antibody (ab155583) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 142 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SNU1 xenograft tissue labeling MAP4K4/NIK with ab155583 at 1/250 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K4/NIK with ab155583 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Strang BL  et al. Identification of lead anti-human cytomegalovirus compounds targeting MAP4K4 via machine learning analysis of kinase inhibitor screening data. PLoS One 13:e0201321 (2018). Read more (PubMed: 30048526) »
  • Wang F  et al. Chemotherapy-induced miR-141/MAP4K4 signaling suppresses progression of colorectal cancer. Biosci Rep 38:N/A (2018). Read more (PubMed: 30429233) »
See all 4 Publications for this product

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