Key features and details
- Rabbit polyclonal to MAP4K4/NIK
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP4K4/NIK antibody
See all MAP4K4/NIK primary antibodies
DescriptionRabbit polyclonal to MAP4K4/NIK
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human MAP4K4/NIK (internal sequence).
- A549 cells. Extracts from COLO and HUVEC cells.
This product was previously labelled as MAP4K4
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 142 kDa (predicted molecular weight: 142 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- FLH21957 antibody
- FLJ10410 antibody
- FLJ20373 antibody
All lanes : Anti-MAP4K4/NIK antibody (ab69885) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from HUVEC cells
Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 142 kDa
Observed band size: 142 kDa
Immunofluorescence analysis of A549 cells using ab69885 at 1/500 dilution, in the presence or absence of the immunising peptide.
ab69885 has not yet been referenced specifically in any publications.