Key features and details
- Rabbit polyclonal to MAP4K4/NIK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP4K4/NIK antibody
See all MAP4K4/NIK primary antibodies
DescriptionRabbit polyclonal to MAP4K4/NIK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human MAP4K4/NIK. (AAO32626.1)
- Whole cell lysate from HeLa cells.
This product was previously labelled as MAP4K4
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSerine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Tissue specificityAppears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- FLH21957 antibody
- FLJ10410 antibody
- FLJ20373 antibody
All lanes : Anti-MAP4K4/NIK antibody (ab80418) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of Human MAP4K4/NIK in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.
ab80418 has been referenced in 1 publication.
- Santhana Kumar K et al. The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma. Springerplus 4:19 (2015). WB ; Human . PubMed: 25625039