Recombinant Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free (ab245752)


  • Product name

    Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free
    See all MAP4K4/NIK primary antibodies
  • Description

    Rabbit monoclonal [SP177] to MAP4K4/NIK - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MAP4K4/NIK aa 850-950 (C terminal). The exact sequence is proprietary.
    Database link: O95819

  • Positive control

    • Human prostate adenocarcinoma tissue.
  • General notes

    ab245752 is the carrier-free version of ab150300 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab245752 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MAP4K4

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab245752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
  • Tissue specificity

    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • FLH21957 antibody
    • FLJ10410 antibody
    • FLJ20373 antibody
    • FLJ90111 antibody
    • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
    • HGK antibody
    • HPK/GCK like kinase antibody
    • HPK/GCK like kinase HGK antibody
    • HPK/GCK-like kinase HGK antibody
    • KIAA0687 antibody
    • M4K4_HUMAN antibody
    • MAP4K4 antibody
    • MAPK/ERK kinase kinase kinase 4 antibody
    • MEK kinase kinase 4 antibody
    • MEKKK 4 antibody
    • MEKKK4 antibody
    • Mitogen activated protein kinase kinase kinase kinase 4 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
    • Nck interacting kinase antibody
    • Nck-interacting kinase antibody
    • NIK antibody
    see all


  • Flow cytometric analysis of rabbit anti-MAP4K4/NIK (SP177) antibody ab150300 (1/400) in HepG2 cells(green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab150300).

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate adenocarcinoma tissue labelling MAP4K4/NIK with ab150300 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab150300).


ab245752 has not yet been referenced specifically in any publications.

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