Key features and details
- Rabbit polyclonal to MAP4K5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP4K5 antibody
DescriptionRabbit polyclonal to MAP4K5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- MOLT4 whole cell lysate; A431 cells; SAS xenograft; 293T, H1299, HepG2 and Raji cell lines
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 95 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
RelevanceMAP4K5, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress. It appears to act upstream of the JUN N-terminal pathway. MAP4K5 interacts with both SH3 domains of the adapter proteins CRK and CRKL. This cytoplasmic protein is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.
- EC 184.108.40.206 antibody
- KHS antibody
- Kinase homologous to SPS1/STE20 antibody
Anti-MAP4K5 antibody (ab96551) at 1/5000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 95 kDa
12% SDS PAGE
ab96551, at 1/200 dilution, staining MAP4K5 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is merged with DNA probe.
ab96551, at 1/100 dilution, staining MAP4K5 in paraffin-embedded SAS xenograft by Immunohistochemistry.
ab96551 has not yet been referenced specifically in any publications.