Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to MAPK 12 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
Product nameAnti-MAPK 12 antibody - BSA and Azide free (Capture)
See all MAPK 12 primary antibodies
DescriptionRabbit monoclonal to MAPK 12 - BSA and Azide free (Capture)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Mouse
ab242582 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse SAPK3. The recommended pair for sandwich ELISA is:
Capture: ab242582, Mouse SAPK3 Capture Antibody (unconjugated)
Detector: ab242828, Mouse SAPK3 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab242582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal to MAPK 12 - BSA and Azide free (Detector) (ab242828).|
FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.
Tissue specificityHighly expressed in skeletal muscle and heart.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Cellular localizationCytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.
- Information by UniProt
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242582 has not yet been referenced specifically in any publications.