Recombinant
RabMAb

Recombinant Anti-MAPK 12 antibody [EPR17637-104] - BSA and Azide free (ab236242)

Overview

  • Product name

    Anti-MAPK 12 antibody [EPR17637-104] - BSA and Azide free
    See all MAPK 12 primary antibodies
  • Description

    Rabbit monoclonal [EPR17637-104] to MAPK 12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse MAPK 12 aa 50-300. The exact sequence is proprietary.
    Database link: O08911

  • Positive control

    • ICC/IF: C2C12 cells.
  • General notes

    Ab236242 is the carrier-free version of ab185145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.
  • Tissue specificity

    Highly expressed in skeletal muscle and heart.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Cellular localization

    Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK 6 antibody
    • ERK-6 antibody
    • ERK6 antibody
    • Extracellular signal-regulated kinase 6 antibody
    • MAP kinase 12 antibody
    • MAP kinase p38 gamma antibody
    • MAPK 12 antibody
    • Mapk12 antibody
    • Mitogen Activated Protein Kinase 12 antibody
    • Mitogen activated protein kinase p38 gamma antibody
    • Mitogen-activated protein kinase 12 antibody
    • Mitogen-activated protein kinase p38 gamma antibody
    • MK12_HUMAN antibody
    • P38 GAMMA antibody
    • P38GAMMA antibody
    • PRKM12 antibody
    • SAPK 3 antibody
    • SAPK-3 antibody
    • SAPK3 antibody
    • Stress Activated Protein Kinase 3 antibody
    • Stress-activated protein kinase 3 antibody
    see all

Images

  • Immunofluorescent analysis of 100% methanol-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling MAPK 12 with ab185145 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in C2C12 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185145).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized C2C12 (mouse myoblast cell line) cell line labeling MAPK 12 with ab185145 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185145).

  • MAPK 12 was immunoprecipitated from 0.35 mg of mouse skeletal muscle lysate with ab185145 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185145 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse skeletal muscle lysate 10 µg (Input). 
    Lane 2: ab185145 IP in mouse skeletal muscle lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185145 in mouse skeletal muscle lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185145).

  • Immunofluorescent analysis of 100% methanol-fixed C2C12 (mouse myoblast cell line) cells labeling MAPK 12 with ab185145 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in C2C12 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185145).

References

ab236242 has not yet been referenced specifically in any publications.

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