Overview

  • Product name

    Anti-MAPK 12 antibody [EPR6528(N)]
    See all MAPK 12 primary antibodies
  • Description

    Rabbit monoclonal [EPR6528(N)] to MAPK 12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human MAPK 12 aa 50-150. The exact sequence is proprietary.
    Database link: P53778

  • Positive control

    • WB: Human skeletal muscle lysate; HeLa, K562, C6 and PC-12 whole cell lysates; Rat heart, spleen and muscle lysates. ICC/IF: A673 and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/500.

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.
  • Tissue specificity

    Highly expressed in skeletal muscle and heart.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Cellular localization

    Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERK 6 antibody
    • ERK-6 antibody
    • ERK6 antibody
    • Extracellular signal-regulated kinase 6 antibody
    • MAP kinase 12 antibody
    • MAP kinase p38 gamma antibody
    • MAPK 12 antibody
    • Mapk12 antibody
    • Mitogen Activated Protein Kinase 12 antibody
    • Mitogen activated protein kinase p38 gamma antibody
    • Mitogen-activated protein kinase 12 antibody
    • Mitogen-activated protein kinase p38 gamma antibody
    • MK12_HUMAN antibody
    • P38 GAMMA antibody
    • P38GAMMA antibody
    • PRKM12 antibody
    • SAPK 3 antibody
    • SAPK-3 antibody
    • SAPK3 antibody
    • Stress Activated Protein Kinase 3 antibody
    • Stress-activated protein kinase 3 antibody
    see all

Images

  • Lane 1: Wild type HAP1 whole cell lysate (0 µg)
    Lane 2: empty knockout HAP1 whole cell lysate (20 µg)
    Lane 3: MAPK 12 whole cell lysate (0 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab205926 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.


  • All lanes : Anti-MAPK 12 antibody [EPR6528(N)] (ab205926) at 1/10000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal skin lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MAPK 12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MAPK 12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 5 : Rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 1 minute; Lane 5: 30 seconds

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A673 (Human muscle Ewing's Sarcoma cell line) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on A673 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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