Recombinant Anti-MAPK 12 antibody [EPR6528(N)] - BSA and Azide free (ab251440)
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- References
- Protocols
Overview
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Product name
Anti-MAPK 12 antibody [EPR6528(N)] - BSA and Azide free
See all MAPK 12 primary antibodies -
Description
Rabbit monoclonal [EPR6528(N)] to MAPK 12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human MAPK 12 aa 50-150. The exact sequence is proprietary.
Database link: P53778 -
General notes
Ab251440 is the carrier-free version of ab205926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab251440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR6528(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab251440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. -
Tissue specificity
Highly expressed in skeletal muscle and heart. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. -
Cellular localization
Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5. - Information by UniProt
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Database links
- Entrez Gene: 6300 Human
- Entrez Gene: 60352 Rat
- Omim: 602399 Human
- SwissProt: P53778 Human
- SwissProt: Q63538 Rat
- Unigene: 432642 Human
- Unigene: 162968 Rat
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Alternative names
- ERK 6 antibody
- ERK-6 antibody
- ERK6 antibody
see all
Datasheets and documents
References
ab251440 has not yet been referenced specifically in any publications.
