Key features and details
- Rabbit monoclonal [E13-Q] to MAPK11 - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAPK11 antibody [E13-Q] - C-terminal
See all MAPK11 primary antibodies
DescriptionRabbit monoclonal [E13-Q] to MAPK11 - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MAPK11 (C terminal). The exact sequence is proprietary.
Database link: Q15759
EpitopeAntibody recognizes the epitope located between Glu352 - Glu363
- Human recombinant MAPK11; HEK293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.32% Tris HCl
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Our Abpromise guarantee covers the use of ab183208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/400 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 41 kDa.|
FunctionKinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
Tissue specificityHighest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
- Information by UniProt
- Human p38Beta MAP kinase mRNA complete cds antibody
- MAP kinase 11 antibody
- MAP kinase p38 beta antibody
Immunofluorescent analysis of HEK293 cells labeling MAPK11 with ab183208 at 1/500 dilution.
All lanes : Anti-MAPK11 antibody [E13-Q] - C-terminal (ab183208) at 1/1000 dilution
Lane 1 : Human recombinant p38-alpha (aa 260-360) with N terminal proprietary tag
Lane 2 : Human recombinant MAPK11 with C terminal DDK tag
Lane 3 : Human recombinant PKC-gamma (aa 1-368) with N terminal proprietary tag
Lane 4 : Human recombinant p38-delta (aa 1-366) with N terminal proprietary tag.
Lysates/proteins at 0.5 µg per lane.
Predicted band size: 41 kDa
ab183208 has been referenced in 1 publication.
- Yu DS et al. Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements. Front Microbiol 9:2724 (2018). PubMed: 30483236