Overview

  • Product name

    Anti-MAPK11 antibody [E13-Q] - C-terminal
    See all MAPK11 primary antibodies
  • Description

    Rabbit monoclonal [E13-Q] to MAPK11 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MAPK11 (C terminal). The exact sequence is proprietary.
    Database link: Q15759

  • Epitope

    Antibody recognizes the epitope located between Glu352 - Glu363
  • Positive control

    • Human recombinant MAPK11; HEK293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.32% Tris HCl
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
  • Clonality

    Monoclonal
  • Clone number

    E13-Q
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/400 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 41 kDa.

Target

  • Function

    Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
  • Tissue specificity

    Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • Human p38Beta MAP kinase mRNA complete cds antibody
    • MAP kinase 11 antibody
    • MAP kinase p38 beta antibody
    • MAPK 11 antibody
    • Mapk11 antibody
    • Mitogen activated protein kinase 11 antibody
    • Mitogen activated protein kinase p38 2 antibody
    • Mitogen activated protein kinase p38 beta antibody
    • Mitogen-activated protein kinase 11 antibody
    • Mitogen-activated protein kinase p38 beta antibody
    • MK11_HUMAN antibody
    • p38 2 antibody
    • p38-2 antibody
    • p38B antibody
    • p38Beta antibody
    • P38BETA2 antibody
    • PRKM11 antibody
    • Protein kinase mitogen activated 11 antibody
    • SAPK2 antibody
    • SAPK2B antibody
    • Stress activated protein kinase 2 antibody
    • Stress activated protein kinase 2b antibody
    • Stress-activated protein kinase 2 antibody
    see all

Images

  • Immunofluorescent analysis of HEK293 cells labeling MAPK11 with ab183208 at 1/500 dilution.

  • All lanes : Anti-MAPK11 antibody [E13-Q] - C-terminal (ab183208) at 1/1000 dilution

    Lane 1 : Human recombinant p38-alpha (aa 260-360) with N terminal proprietary tag
    Lane 2 : Human recombinant MAPK11 with C terminal DDK tag
    Lane 3 : Human recombinant PKC-gamma (aa 1-368) with N terminal proprietary tag
    Lane 4 : Human recombinant p38-delta (aa 1-366) with N terminal proprietary tag.

    Lysates/proteins at 0.5 µg per lane.

    Predicted band size: 41 kDa

References

This product has been referenced in:

  • Yu DS  et al. Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements. Front Microbiol 9:2724 (2018). Read more (PubMed: 30483236) »
See 1 Publication for this product

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