Key features and details
- Rabbit polyclonal to MAPK4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAPK4 antibody
See all MAPK4 primary antibodies
DescriptionRabbit polyclonal to MAPK4
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human MAPK4 aa 1-326. The exact sequence is proprietary. (NP_002738.2).
Database link: P31152
- H1299 whole cell lysate; Human small intestine tissue; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 89% Tris glycine
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
FunctionAtypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.
Tissue specificityHigh expression in heart and brain.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe FRIEDE motif is required for docking MAPKAPK5.
In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
modificationsPhosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Cellular localizationCytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
- Information by UniProt
- ERK-4 antibody
- ERK3 antibody
- Erk3 related antibody
Anti-MAPK4 antibody (ab211501) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 66 kDa
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAPK4 with ab211501 at 1/200 dilution. The lower panel is merged with a DNA probe.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling MAPK4 with ab211501 at 7.5 µg/ml.
ab211501 has not yet been referenced specifically in any publications.