Overview

  • Product name

    Anti-MAPK6/ERK-3 antibody [EP1720Y]
    See all MAPK6/ERK-3 primary antibodies
  • Description

    Rabbit monoclonal [EP1720Y] to MAPK6/ERK-3
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAPK6/ERK-3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16659

  • Positive control

    • WB: HeLa whole cell lysate (ab150035), Rat brain, and C2C12 lysates. IHC-P: human kidney carcinoma and human breast carcinoma tissue.
  • General notes

    Previously labelled as MAPK6.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab53277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
    • Tissue specificity

      Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications

      Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
    • Information by UniProt
    • Database links

    • Alternative names

      • ERK-3 antibody
      • ERK3 antibody
      • Extracellular signal regulated kinase 3 antibody
      • Extracellular signal regulated kinase p97 antibody
      • Extracellular signal-regulated kinase 3 antibody
      • HsT17250 antibody
      • MAP kinase 6 antibody
      • MAP kinase isoform p97 antibody
      • MAPK 6 antibody
      • MAPK6 antibody
      • Mitogen activated protein kinase 6 antibody
      • Mitogen-activated protein kinase 6 antibody
      • MK06_HUMAN antibody
      • p97 MAPK antibody
      • p97-MAPK antibody
      • PRKM6 antibody
      • Protein kinase mitogen activated 5 antibody
      • Protein kinase mitogen activated 6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling MAPK6/ERK-3 with Purified ab53277 at 1:100 dilution (13.52 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-MAPK6/ERK-3 antibody [EP1720Y] (ab53277) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Rat brain lysates
      Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 63 kDa
      Observed band size: 105 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in PMID:30642948 and 30166347

    • Unpurified ab53277 (1:50) staining human MAPK6/ERK-3 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Ronkina N  et al. Germ Line Deletion Reveals a Nonessential Role of Atypical Mitogen-Activated Protein Kinase 6/Extracellular Signal-Regulated Kinase 3. Mol Cell Biol 39:N/A (2019). Read more (PubMed: 30642948) »
    • Chen M  et al. The atypical MAPK ERK3 potently suppresses melanoma cell growth and invasiveness. J Cell Physiol N/A:N/A (2018). Read more (PubMed: 30569573) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    melanoma cell line
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

    Dr. 令 中野

    Verified customer

    Submitted Jun 20 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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