• Product name

    Anti-MAPKAPK2/3 antibody
  • Description

    Rabbit polyclonal to MAPKAPK2/3
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 332-336 of Human MAPKAPK2

  • Positive control

    • Recombinant proteins



Our Abpromise guarantee covers the use of ab82543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 , 45 kDa.


  • Relevance

    MAPKAPK2 (MAP kinase activated protein kinase 2, MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha. MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • 3PK antibody
    • AA960234 antibody
    • MAP kinase activated protein kinase 2 antibody
    • MAP kinase activated protein kinase 3 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAP kinase 3 antibody
    • MK2 antibody
    • MK3 antibody
    • OTTHUMP00000034531 antibody
    • Rps6kc1 antibody
    see all


  • All lanes : Anti-MAPKAPK2/3 antibody (ab82543) at 1/1000 dilution

    Lane 1 : Human recombinant MAPKAPK2 with proprietary tag
    Lane 2 : human recombinant MAPKAPK3 with proprietary tag

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 43 , 45 kDa
    Observed band size: 65,70 kDa
    why is the actual band size different from the predicted?

    The higher observed MW is due to the presence of the tag.


ab82543 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82543.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up