Product nameAnti-MAPRE1 antibody [KT51]
See all MAPRE1 primary antibodies
DescriptionRat monoclonal [KT51] to MAPRE1
Tested applicationsSuitable for: IP, ELISA, WB, ICC/IF, Flow Cyt, ICCmore details
Species reactivityReacts with: Mouse, Hamster, Human
The C terminal part of Mouse MAPRE1 (amino acids 125-268) fused to a tag, purified from E. coli BL21 bacteria.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/7000. Predicted molecular weight: 30 kDa.|
|ICC/IF||1/100 - 1/1000.|
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.
DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
- Information by UniProt
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
ab53358 staining MAPRE1 in Hela cells (red). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained both with DAPI - to highlight the nucleus (blue) - and an anti-tubulin antibody (green). Please refer to abreview for further experimental details.
Anti-MAPRE1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µg
Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 5 seconds
This product has been referenced in:
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). Read more (PubMed: 28994651) »
- Rivaud MR et al. Sodium Channel Remodeling in Subcellular Microdomains of Murine Failing Cardiomyocytes. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 29222390) »