Anti-MAPRE1/EB1 antibody (ab50188)
Key features and details
- Rabbit polyclonal to MAPRE1/EB1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MAPRE1/EB1 antibody
See all MAPRE1/EB1 primary antibodies -
Description
Rabbit polyclonal to MAPRE1/EB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIP HumanWB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human MAPRE1/EB1 aa 252-268 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:GFVIPDEGGPQEEQEEY
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Positive control
- Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.
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General notes
This product was previously labelled as MAPRE1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab50188 is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| IP |
Human
|
| WB |
Mouse
Rat
Human
|
| All applications |
Rat
Chicken
Cow
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF | (3) |
| Notes |
|---|
IP: 10-20 ug of the antibody can immunoprecipitate EB1 from a cell lysate of HeLa cells.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. Staining of the EB1 band in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 419288 Chicken
- Entrez Gene: 507845 Cow
- Entrez Gene: 22919 Human
- Entrez Gene: 13589 Mouse
- Entrez Gene: 114764 Rat
- Omim: 603108 Human
- SwissProt: Q5ZLC7 Chicken
- SwissProt: Q3ZBD9 Cow
see all -
Alternative names
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
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Western blot - Anti-MAPRE1/EB1 antibody (ab50188)All lanes : Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : Rat1 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : SW48 cell lysate
Lane 6 : HCT116 cell lysate
Lane 7 : HEK293 cell lysate
Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
50kDa band represents Rb IgG heavy chain -
Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain
Lane 1 : No primary antibody
Lanes 2-4 : Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg
Lanes 1-2 : No lysate
Lane 3 : HeLa cell lysate at 100 µg
Lane 4 : HeLa cell lysate at 25 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab50188 has been referenced in 6 publications.
- Logan CM et al. Functional role for stable microtubules in lens fiber cell elongation. Exp Cell Res 362:477-488 (2018). PubMed: 29253534
- Almada E et al. Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures. Sci Rep 7:14894 (2017). PubMed: 29097729
- Shimozawa T et al. Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging. Proc Natl Acad Sci U S A 110:3399-404 (2013). ICC/IF ; Human . PubMed: 23401517
- Goldspink DA et al. The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation. J Cell Sci 126:4000-14 (2013). PubMed: 23813963
- Nakamura S et al. Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells. PLoS One 7:e51442 (2012). WB ; Human . PubMed: 23251535
- Brüning-Richardson A et al. EB1 is required for spindle symmetry in mammalian mitosis. PLoS One 6:e28884 (2011). PubMed: 22216133