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    mapre1eb1-antibody-ab50188.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-MAPRE1/EB1 antibody (ab50188)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)
  • Western blot - Anti-MAPRE1/EB1 antibody (ab50188)
  • Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)

Key features and details

  • Rabbit polyclonal to MAPRE1/EB1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MAPRE1/EB1 antibody
    See all MAPRE1/EB1 primary antibodies
  • Description

    Rabbit polyclonal to MAPRE1/EB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human MAPRE1/EB1 aa 252-268 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    GFVIPDEGGPQEEQEEY

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.
  • General notes

     This product was previously labelled as MAPRE1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab50188 is affinity-purified using the immunizing peptide immobilized on agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab50188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
IP
Human
WB
Mouse
Rat
Human
All applications
Rat
Chicken
Cow
Dog
Pig
Application Abreviews Notes
WB
IP
ICC/IF (3)
Notes
  • Application notes
    ICC/IF: Use at a concentration of 1 - 2 µg/ml.
    IP: 10-20 ug of the antibody can immunoprecipitate EB1 from a cell lysate of HeLa cells.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. Staining of the EB1 band in immunoblotting is specifically inhibited with the immunizing peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the MAPRE family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 EB1 C-terminal domain.
    • Domain

      Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
    • Target information above from: UniProt accession Q15691 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 419288 Chicken
      • Entrez Gene: 507845 Cow
      • Entrez Gene: 22919 Human
      • Entrez Gene: 13589 Mouse
      • Entrez Gene: 114764 Rat
      • Omim: 603108 Human
      • SwissProt: Q5ZLC7 Chicken
      • SwissProt: Q3ZBD9 Cow
      • SwissProt: Q15691 Human
      • SwissProt: Q61166 Mouse
      • SwissProt: Q66HR2 Rat
      • Unigene: 472437 Human
      • Unigene: 143877 Mouse
      • Unigene: 7652 Rat
      see all
    • Alternative names

      • 5530600P05Rik antibody
      • Adenomatous polyposis coli binding protein EB 1 antibody
      • Adenomatous polyposis coli binding protein EB1 antibody
      • AI462499 antibody
      • AI504412 antibody
      • APC binding protein EB 1 antibody
      • APC binding protein EB1 antibody
      • APC-binding protein EB1 antibody
      • AW260097 antibody
      • BIM1p antibody
      • D2Ertd459e antibody
      • EB 1 antibody
      • EB1 antibody
      • End binding protein 1 antibody
      • End-binding protein 1 antibody
      • fa01e12 antibody
      • fc23e11 antibody
      • fi33c06 antibody
      • MAPRE 1 antibody
      • MAPRE1 antibody
      • Mapre3 antibody
      • MARE1_HUMAN antibody
      • MGC117374 antibody
      • MGC129946 antibody
      • MGC52508 antibody
      • Microtubule associated protein RP/EB family member 1 antibody
      • Microtubule-associated protein RP/EB family member 1 antibody
      • wu:fa01e12 antibody
      • wu:fc23e11 antibody
      • wu:fi33c06 antibody
      • zgc:55428 antibody
      • zgc:77807 antibody
      • zgc:85755 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)
      Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)
      ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
    • Western blot - Anti-MAPRE1/EB1 antibody (ab50188)
      Western blot - Anti-MAPRE1/EB1 antibody (ab50188)
      All lanes : Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/ml

      Lane 1 : HeLa cell lysate

      Lane 2 : NIH3T3 cell lysate

      Lane 3 : Rat1 cell lysate

      Lane 4 : A431 cell lysate
      Lane 5 : SW48 cell lysate
      Lane 6 : HCT116 cell lysate
      Lane 7 : HEK293 cell lysate

      Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml

      Secondary
      All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.



      50kDa band represents Rb IgG heavy chain
    • Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)
      Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)
      RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain

      Lane 1 : No primary antibody
      Lanes 2-4 : Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg

      Lanes 1-2 : No lysate
      Lane 3 : HeLa cell lysate at 100 µg
      Lane 4 : HeLa cell lysate at 25 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

      Observed band size: 34 kDa why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab50188? Please let us know so that we can cite the reference in this datasheet.

    ab50188 has been referenced in 6 publications.

    • Logan CM  et al. Functional role for stable microtubules in lens fiber cell elongation. Exp Cell Res 362:477-488 (2018). PubMed: 29253534
    • Almada E  et al. Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures. Sci Rep 7:14894 (2017). PubMed: 29097729
    • Shimozawa T  et al. Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging. Proc Natl Acad Sci U S A 110:3399-404 (2013). ICC/IF ; Human . PubMed: 23401517
    • Goldspink DA  et al. The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation. J Cell Sci 126:4000-14 (2013). PubMed: 23813963
    • Nakamura S  et al. Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells. PLoS One 7:e51442 (2012). WB ; Human . PubMed: 23251535
    • Brüning-Richardson A  et al. EB1 is required for spindle symmetry in mammalian mitosis. PLoS One 6:e28884 (2011). PubMed: 22216133

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAPRE1/EB1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (MDCK cysts)
    Permeabilization
    Yes - Tritón 0.3 %
    Specification
    MDCK cysts
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 16°C
    Fixative
    Methanol
    Read More

    Dr. M. Cecilia Larocca

    Verified customer

    Submitted Jan 28 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAPRE1/EB1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH 3T3)
    Permeabilization
    Yes - Triton X-100
    Specification
    NIH 3T3
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Glutaraldehyde + Formaldehyde
    Read More

    Mr. Ian Kent

    Verified customer

    Submitted Dec 12 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAPRE1/EB1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted May 08 2008

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