Anti-MAPRE1/EB1 antibody (ab50188)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-MAPRE1/EB1 antibody
See all MAPRE1/EB1 primary antibodies -
Description
Rabbit polyclonal to MAPRE1/EB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human MAPRE1/EB1 aa 252-268 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:GFVIPDEGGPQEEQEEY
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Positive control
- Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.
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General notes
This product was previously labelled as MAPRE1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab50188 is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab50188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
Application notes
ICC/IF: Use at a concentration of 1 - 2 µg/ml.
IP: 10-20 ug of the antibody can immunoprecipitate EB1 from a cell lysate of HeLa cells.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. Staining of the EB1 band in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 10-20 ug of the antibody can immunoprecipitate EB1 from a cell lysate of HeLa cells.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. Staining of the EB1 band in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 419288 Chicken
- Entrez Gene: 507845 Cow
- Entrez Gene: 22919 Human
- Entrez Gene: 13589 Mouse
- Entrez Gene: 114764 Rat
- Omim: 603108 Human
- SwissProt: Q5ZLC7 Chicken
- SwissProt: Q3ZBD9 Cow
see all -
Alternative names
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000. -
Western blot - Anti-MAPRE1/EB1 antibody (ab50188)All lanes : Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : Rat1 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : SW48 cell lysate
Lane 6 : HCT116 cell lysate
Lane 7 : HEK293 cell lysate
Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
50kDa band represents Rb IgG heavy chain -
Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain
Lane 1 : No primary antibody
Lanes 2-4 : Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg
Lanes 1-2 : No lysate
Lane 3 : HeLa cell lysate at 100 µg
Lane 4 : HeLa cell lysate at 25 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Protocols
References
This product has been referenced in:
- Logan CM et al. Functional role for stable microtubules in lens fiber cell elongation. Exp Cell Res 362:477-488 (2018). Read more (PubMed: 29253534) »
- Almada E et al. Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures. Sci Rep 7:14894 (2017). Read more (PubMed: 29097729) »
