Product nameAnti-MAPRE1/EB1 antibody [KT51]
See all MAPRE1/EB1 primary antibodies
DescriptionRat monoclonal [KT51] to MAPRE1/EB1
Tested applicationsSuitable for: IP, ELISA, WB, ICC/IF, Flow Cyt, ICCmore details
Species reactivityReacts with: Mouse, Hamster, Human
Synthetic peptide corresponding to Mouse MAPRE1/EB1 aa 125-268 (C terminal).
This product was previously labelled as MAPRE1
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/7000. Predicted molecular weight: 30 kDa.|
|ICC/IF||1/100 - 1/1000.|
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.
DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
- Information by UniProt
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
ab53358 staining MAPRE1/EB1 in Hela cells (red). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained both with DAPI - to highlight the nucleus (blue) - and an anti-tubulin antibody (green). Please refer to abreview for further experimental details.
Anti-MAPRE1/EB1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µg
Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 5 seconds
Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This product has been referenced in:
- Ivashko-Pachima Y et al. NAP (davunetide) preferential interaction with dynamic 3-repeat Tau explains differential protection in selected tauopathies. PLoS One 14:e0213666 (2019). Read more (PubMed: 30865715) »
- Chang CL et al. EB1 binding restricts STIM1 translocation to ER-PM junctions and regulates store-operated Ca2+ entry. J Cell Biol 217:2047-2058 (2018). Read more (PubMed: 29563214) »