Overview

  • Product name
    Anti-MAPRE1/EB1 antibody [KT51]
    See all MAPRE1/EB1 primary antibodies
  • Description
    Rat monoclonal [KT51] to MAPRE1/EB1
  • Host species
    Rat
  • Tested applications
    Suitable for: IP, ELISA, WB, ICC/IF, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse MAPRE1/EB1 aa 125-268 (C terminal).

  • General notes

     This product was previously labelled as MAPRE1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab53358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/7000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

Target

  • Function
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • Domain
    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5530600P05Rik antibody
    • Adenomatous polyposis coli binding protein EB 1 antibody
    • Adenomatous polyposis coli binding protein EB1 antibody
    • AI462499 antibody
    • AI504412 antibody
    • APC binding protein EB 1 antibody
    • APC binding protein EB1 antibody
    • APC-binding protein EB1 antibody
    • AW260097 antibody
    • BIM1p antibody
    • D2Ertd459e antibody
    • EB 1 antibody
    • EB1 antibody
    • End binding protein 1 antibody
    • End-binding protein 1 antibody
    • fa01e12 antibody
    • fc23e11 antibody
    • fi33c06 antibody
    • MAPRE 1 antibody
    • MAPRE1 antibody
    • Mapre3 antibody
    • MARE1_HUMAN antibody
    • MGC117374 antibody
    • MGC129946 antibody
    • MGC52508 antibody
    • Microtubule associated protein RP/EB family member 1 antibody
    • Microtubule-associated protein RP/EB family member 1 antibody
    • wu:fa01e12 antibody
    • wu:fc23e11 antibody
    • wu:fi33c06 antibody
    • zgc:55428 antibody
    • zgc:77807 antibody
    • zgc:85755 antibody
    see all

Images

  • ab53358 staining MAPRE1/EB1 in Hela cells (red). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained both with DAPI - to highlight the nucleus (blue) - and an anti-tubulin antibody (green). Please refer to abreview for further experimental details.

    See Abreview

  • Anti-MAPRE1/EB1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µg

    Secondary
    Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa


    Exposure time: 5 seconds
  • Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
  • Ivashko-Pachima Y  et al. NAP (davunetide) preferential interaction with dynamic 3-repeat Tau explains differential protection in selected tauopathies. PLoS One 14:e0213666 (2019). Read more (PubMed: 30865715) »
  • Chang CL  et al. EB1 binding restricts STIM1 translocation to ER-PM junctions and regulates store-operated Ca2+ entry. J Cell Biol 217:2047-2058 (2018). Read more (PubMed: 29563214) »
See all 10 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 22 2011

Application
Immunocytochemistry
Sample
Human Cultured Cells (HELA)
Permeabilization
Yes - 0.5% Triton-X
Specification
HELA
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Feb 03 2011

Application
Western blot
Sample
Human Cell lysate - other (HELA)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HELA
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 03 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 05 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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