Key features and details
- Rabbit polyclonal to MARCH2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MARCH2 antibody
See all MARCH2 primary antibodies
DescriptionRabbit polyclonal to MARCH2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human colon tissue; MARCH2 overexpression HEK293T lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.|
FunctionE3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.
Tissue specificityBroadly expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-CH-type zinc finger.
DomainThe RING-CH-type zinc finger domain is required for E3 ligase activity.
Cellular localizationEndoplasmic reticulum membrane. Lysosome membrane. Endosome membrane.
- Information by UniProt
- E3 ubiquitin protein ligase MARCH2 antibody
- E3 ubiquitin-protein ligase MARCH2 antibody
- EC 6.3.2.- antibody
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MARCH2 with ab220292 at 1/20 dilution.
All lanes : Anti-MARCH2 antibody (ab220292) at 1/100 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : MARCH2 overexpression HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 27 kDa
ab220292 has not yet been referenced specifically in any publications.