Overview

  • Product name

  • Description

    Rabbit polyclonal to MARCH3
  • Host species

    Rabbit
  • Specificity

    ab137261 detects endogenous levels of total MARCH3 protein.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human MARCH3 aa 111-160 (internal sequence).
    Sequence:

    SYCELCHFRFAVERKPRPLV EWLRNPGPQH EKRTLFGDMV CFLFITPLAT


    Database link: Q86UD3

  • Positive control

    • Raw264.7 cell lysate, K562 and COS7 cell lines, human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-CH-type zinc finger.
  • Domain

    The RING-CH-type zinc finger domain is required for E3 ligase activity.
  • Cellular localization

    Cytoplasmic vesicle membrane. Early endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase MARCH3 antibody
    • E3 ubiquitin-protein ligase MARCH3 antibody
    • FLJ20096 antibody
    • March 3 antibody
    • MARCH III antibody
    • MARCH-III antibody
    • March3 antibody
    • MARH3_HUMAN antibody
    • Membrane associated RING CH protein III antibody
    • Membrane associated ring finger (C3HC4) 3 antibody
    • Membrane associated ring finger (C3HC4) 3, E3 ubiquitin protein ligase antibody
    • Membrane associated RING finger protein 3 antibody
    • Membrane-associated RING finger protein 3 antibody
    • Membrane-associated RING-CH protein III antibody
    • MGC48332 antibody
    • RING finger protein 173 antibody
    • RNF173 antibody
    see all

Images

  • Anti-MARCH3 antibody (ab137261) at 1/500 dilution + Raw264.7 cell lysate at 30 µg

    Predicted band size: 29 kDa

  • All lanes : Anti-MARCH3 antibody (ab137261) at 1/500 dilution

    Lane 1 : K562 cell line
    Lane 2 : COS7 cell line
    Lane 3 : K562 cell line with Blocking with synthesized peptide

    Predicted band size: 29 kDa

  • Immunohistochemistry analysis of paraffin-embedded human heart tissue, using ab137261 at 1:50 dilution. The picture on the right is blocked with the synthesized peptide.

References

ab137261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137261.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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