Key features and details
- Rabbit polyclonal to MARCH3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MARCH3 antibody
See all MARCH3 primary antibodies
DescriptionRabbit polyclonal to MARCH3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human brain cortex tissue; K562 and COS7 cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
FunctionE3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-CH-type zinc finger.
DomainThe RING-CH-type zinc finger domain is required for E3 ligase activity.
Cellular localizationCytoplasmic vesicle membrane. Early endosome membrane.
- Information by UniProt
- E3 ubiquitin protein ligase MARCH3 antibody
- E3 ubiquitin-protein ligase MARCH3 antibody
- FLJ20096 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling MARCH3 with ab190219 at 10 µg/ml.
All lanes : Anti-MARCH3 antibody (ab190219) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : COS7 cell extract
Lane 3 : K562 cell extract with immunizing peptide
Predicted band size: 29 kDa
ab190219 has not yet been referenced specifically in any publications.