Product nameAnti-MARCKS antibody
See all MARCKS primary antibodies
DescriptionRabbit polyclonal to MARCKS
Specificityab51100 detects endogenous levels of total MARCKS protein.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthentic non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).
- Human brain tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab51100 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Corresponding phospho antibody
Our Abpromise guarantee covers the use of ab51100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Incubate for 1 hour.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Sequence similaritiesBelongs to the MARCKS family.
modificationsPhosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Cellular localizationCytoplasm > cytoskeleton. Membrane.
- Information by UniProt
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
ab51100 at 1/100 dilution staining MARCKS in paraffin embedded human brain sections by Immunohistochemistry, in the absence and presence of the immunizing (blocking) peptide.
Anti-MARCKS antibody (ab51100) at 1/2000 dilution + 293 cells, lysed in RIPA buffer containing minicomplete protease inhibitors at 30 µg
HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Primary antibody was incubated with the blot for 1 hour at 20°C. Run on neutral pH gel which may account for unexpectedly low MW due to charge effects.
This product has been referenced in:
- Wang Y et al. Adipokine RBP4 drives ovarian cancer cell migration. J Ovarian Res 11:29 (2018). Read more (PubMed: 29642915) »
- Song HM et al. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS. Int J Mol Med 42:193-207 (2018). Read more (PubMed: 29620148) »