Product nameAnti-MARCKS (phospho S158) antibody [EP2113Y] - BSA and Azide free
See all MARCKS primary antibodies
DescriptionRabbit monoclonal [EP2113Y] to MARCKS (phospho S158) - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MARCKS. The exact sequence is proprietary.
- WB: HEK293 cells treated with TPA.
Ab239900 is the carrier-free version of ab81295. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239900 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.|
FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Sequence similaritiesBelongs to the MARCKS family.
modificationsPhosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Cellular localizationCytoplasm > cytoskeleton. Membrane.
- Information by UniProt
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution
All lanes : HEK293 cells treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81295).
ab239900 has not yet been referenced specifically in any publications.