Anti-MARCKS (phospho S162) antibody (ab194804)
Key features and details
- Rabbit polyclonal to MARCKS (phospho S162)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MARCKS (phospho S162) antibody
See all MARCKS primary antibodies -
Description
Rabbit polyclonal to MARCKS (phospho S162) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MARCKS (phospho S162). A phospho specific peptide corresponding to residues surrounding S162 of Human MARCKS.
Database link: P29966 -
Positive control
- PMA-treated 293 cell extract; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
Sequence similarities
Belongs to the MARCKS family. -
Post-translational
modificationsPhosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
Cellular localization
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4082 Human
- Omim: 177061 Human
- SwissProt: P29966 Human
- Unigene: 519909 Human
- Unigene: 712721 Human
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Alternative names
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
Images
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All lanes : Anti-MARCKS (phospho S162) antibody (ab194804) at 1/500 dilution
Lane 1 : Untreated 293 cell extract
Lane 2 : PMA-treated 293 cell extract
Predicted band size: 32 kDa -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling MARCKS (phospho S162) (red) with ab194804 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab194804 has not yet been referenced specifically in any publications.