Key features and details
- Rabbit polyclonal to MARCKS (phospho S162)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MARCKS (phospho S162) antibody
See all MARCKS primary antibodies
DescriptionRabbit polyclonal to MARCKS (phospho S162)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MARCKS (phospho S162). A phospho specific peptide corresponding to residues surrounding S162 of Human MARCKS.
Database link: P29966
- PMA-treated 293 cell extract; HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Sequence similaritiesBelongs to the MARCKS family.
modificationsPhosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Cellular localizationCytoplasm > cytoskeleton. Membrane.
- Information by UniProt
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
All lanes : Anti-MARCKS (phospho S162) antibody (ab194804) at 1/500 dilution
Lane 1 : Untreated 293 cell extract
Lane 2 : PMA-treated 293 cell extract
Predicted band size: 32 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling MARCKS (phospho S162) (red) with ab194804 at 1/100 dilution.
ab194804 has not yet been referenced specifically in any publications.