Anti-Mare antibody (ab121346)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Mare antibody
See all Mare primary antibodies -
Description
Rabbit polyclonal to Mare -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human Mare aa 60-199.
Database link: Q12980 -
Positive control
- IHC-P: Human skin, endometrium and prostate tissue. WB: RT-4 and NBT-II cell lysate.
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General notes
This product was previously labelled as NPRL3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use citrate buffer pH 6. | |
| WB | 1/250 - 1/500. |
Target
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Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the NPR3 family. - Information by UniProt
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Database links
- Entrez Gene: 8131 Human
- Entrez Gene: 17168 Mouse
- Entrez Gene: 360505 Rat
- Omim: 600928 Human
- SwissProt: Q12980 Human
- SwissProt: Q8VIJ8 Mouse
- Unigene: 19699 Human
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Alternative names
- -14 gene protein antibody
- Alpha-globin regulatory element-containing gene protein antibody
- C16ORF35 antibody
see all
Images
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Western blot - Anti-Mare antibody (ab121346)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mare antibody (ab121346)Paraffin-embedded human endometrium tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Mare antibody (ab121346)Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121346 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mare antibody (ab121346)Paraffin-embedded human prostate tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mare antibody (ab121346)ab121346, at 1/50 dilution, staining NRPL3 in paraffin-embedded Human skin tissue by Immunohistochemistry. -
Western blot - Anti-Mare antibody (ab121346)All lanes : Anti-Mare antibody (ab121346)
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
References
ab121346 has not yet been referenced specifically in any publications.
