Product nameAnti-Mare antibody
See all Mare primary antibodies
DescriptionRabbit polyclonal to Mare
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human Mare aa 60-199.
Database link: Q12980
- IHC-P: Human skin, endometrium and prostate tissue. WB: RT-4 and NBT-II cell lysate.
This product was previously labelled as NPRL3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use citrate buffer pH 6.|
|WB||1/250 - 1/500.|
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the NPR3 family.
- Information by UniProt
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Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Paraffin-embedded human endometrium tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121346 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Paraffin-embedded human prostate tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
ab121346, at 1/50 dilution, staining NRPL3 in paraffin-embedded Human skin tissue by Immunohistochemistry.
All lanes : Anti-Mare antibody (ab121346)
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
Developed using the ECL technique.
ab121346 has not yet been referenced specifically in any publications.