Anti-Mare antibody (ab121346)
Key features and details
- Rabbit polyclonal to Mare
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mare antibody
See all Mare primary antibodies -
Description
Rabbit polyclonal to Mare -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human Mare aa 60-199.
Database link: Q12980 -
Positive control
- IHC-P: Human endometrium and prostate tissue. ICC: A431 cells.
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General notes
This product was previously labelled as NPRL3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the NPR3 family. - Information by UniProt
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Database links
- Entrez Gene: 8131 Human
- Entrez Gene: 17168 Mouse
- Entrez Gene: 360505 Rat
- Omim: 600928 Human
- SwissProt: Q12980 Human
- SwissProt: Q8VIJ8 Mouse
- Unigene: 19699 Human
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Alternative names
- -14 gene protein antibody
- Alpha-globin regulatory element-containing gene protein antibody
- C16ORF35 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mare antibody (ab121346)
Paraffin-embedded human endometrium tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
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Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121346 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mare antibody (ab121346)
Paraffin-embedded human prostate tissue staining MARE with ab121346 at 1/50 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
References (1)
ab121346 has been referenced in 1 publication.
- Yuskaitis CJ et al. Chronic mTORC1 inhibition rescues behavioral and biochemical deficits resulting from neuronal Depdc5 loss in mice. Hum Mol Genet 28:2952-2964 (2019). PubMed: 31174205