Key features and details
- Rabbit polyclonal to MARK1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MARK1 antibody - N-terminal
See all MARK1 primary antibodies
DescriptionRabbit polyclonal to MARK1 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
Recombinant fragment corresponding to a region within N terminal amino acids 1-265 of Human MARK1 (Uniprot ID Q9P0L2)
- A431 whole cell lysate, H1299 whole cell lysate, HeLa whole cell lysate and HeLa paraformaldehyde-fixed cells
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 89 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay play a role in cytoskeletal stability.
Tissue specificityHighly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cellular localizationCytoplasm > cytoskeleton. Appears to localize to an intracellular network.
- Information by UniProt
- KIAA1477 antibody
- MAP/microtubule affinity regulating kinase 1 antibody
- MAP/microtubule affinity-regulating kinase 1 antibody
Anti-MARK1 antibody - N-terminal (ab154357) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MARK1 with ab154357 at 1/200 dilution (green). Bottom panel shows image merged with DNA probe (blue).
ab154357 has not yet been referenced specifically in any publications.