Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5463] to MARK1 (phospho T215)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-MARK1 (phospho T215) antibody [EPR5463]
See all MARK1 primary antibodies
DescriptionRabbit monoclonal [EPR5463] to MARK1 (phospho T215)
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide corresponding to residues surrounding Threonine 215 of Human MARK1 (UniProt Q9P0L2).
- SH-SY5Y cell lysates, treated with Okadaic acid and Calyculin A.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.62 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).|
FunctionMay play a role in cytoskeletal stability.
Tissue specificityHighly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cellular localizationCytoplasm > cytoskeleton. Appears to localize to an intracellular network.
- Information by UniProt
- KIAA1477 antibody
- MAP/microtubule affinity regulating kinase 1 antibody
- MAP/microtubule affinity-regulating kinase 1 antibody
All lanes : Anti-MARK1 (phospho T215) antibody [EPR5463] (ab126731) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126731 has not yet been referenced specifically in any publications.