Recombinant Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antib (ab248152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5463] to MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) - BSA and Azide free
- Suitable for: Dot blot, WB, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antib -
Description
Rabbit monoclonal [EPR5463] to MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects MARK1 phosphorylated at Threonine 215.
-
Tested applications
Suitable for: Dot blot, WB, ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab248152 is the carrier-free version of ab126731.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.62 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5463 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
|
|
ELISA |
Use at an assay dependent concentration.
|
Notes |
---|
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa). |
ELISA
Use at an assay dependent concentration. |
Target
-
Cellular localization
MARK4: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus. MARK2: Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. MARK1: Cytoplasm > cytoskeleton. Appears to localize to an intracellular network. -
Database links
- Entrez Gene: 2011 Human
- Entrez Gene: 4139 Human
- Entrez Gene: 57787 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 226778 Mouse
- Entrez Gene: 232944 Mouse
- Entrez Gene: 117016 Rat
- Entrez Gene: 60328 Rat
see all
Images
-
Westernblot analysis of ab126731 at a 1/20 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as a scondary antibody at 1/20000 dilution.
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate 15μg
Lane 2: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 3: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 5: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 6: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 7: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 8: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 9: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Exposure time:
Lane 1 – 3, 6 - 9: 180 seconds
Lane 4 - 5: 7 seconds
Endogenous MARK proteins are observed at ~110 kDa reported by PMID: 9108484 and 16238695
-
Indirect ELISA antibody dose-response curve using ab126731 between 0-10000 ng/ml. Antigen concentration of 500 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
-
Dot Blot using ab126731 at a 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution
Lane 1: Human MARK1 (T215) phospho peptide
Lane 2: Human MARK1 non-phospho peptide
Lane 3: Human MARK2 (T208) phospho peptide
Lane 4: Human MARK2 non-phospho peptide
Lane 5: Human MARK3 (T234) phosphor peptide
Lane 6: Human MARK3 non-phospho peptide
Lane 7: Human MARK4 (T214) phospho peptide
Lane 8: Human MARK4 non-phospho peptide
-
All lanes : Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antib (ab126731) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDaThis data was developed using ab126731, the same antibody clone in a different buffer formulation.
-
This data was developed using ab126731, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab248152 has not yet been referenced specifically in any publications.