Key features and details
- Rabbit polyclonal to MARK2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MARK2 antibody
See all MARK2 primary antibodies
DescriptionRabbit polyclonal to MARK2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region within amino acids 350-400 of Human MARK2 (NP_001034558.2).
- 293T and Jurkat whole cell lysates
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH range: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab113284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 88 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
- Information by UniProt
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
All lanes : Anti-MARK2 antibody (ab113284) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 3 minutes
ab113284 at 1 µg/ml staining MARK2 in 293T whole cell lysate immunoprecipitated using ab113284 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 88 kDa. Exposure time: 30 seconds.
ab113284 has not yet been referenced specifically in any publications.