Key features and details
- Goat polyclonal to MARK2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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- Success from the first experiment – confirmed specificity through extensive validation
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Product nameAnti-MARK2 antibody
See all MARK2 primary antibodies
DescriptionGoat polyclonal to MARK2
SpecificityExpected to recognize both reported isoforms b and c (NP_004945.3 and NP_001034557.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human Brain (Cerebellum) lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
- Information by UniProt
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
Anti-MARK2 antibody (ab77641) at 0.3 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg
Predicted band size: 77 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab77641 has been referenced in 2 publications.
- McRae R et al. Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells. Cell Logist 7:e1271498 (2017). Dog, Human . PubMed: 28396819
- Lázaro-Diéguez F et al. Par1b links lumen polarity with LGN-NuMA positioning for distinct epithelial cell division phenotypes. J Cell Biol 203:251-64 (2013). PubMed: 24165937
Customer reviews and Q&As
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