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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MARK
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MARK2 antibody [EPR8553] (ab133724)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)
  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Anti-MARK2 antibody [EPR8553] (ab133724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8553] to MARK2
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

You may also be interested in

Protein
Product image
Recombinant human MARK2 protein (ab119135)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MARK2 antibody [EPR8553]
    See all MARK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8553] to MARK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human MARK2 (Q7KZI7).

  • Positive control

    • Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.70 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8553
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MARK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Alternative Versions

    • Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human MARK2 protein (ab119135)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
    • Tissue specificity

      High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Cellular localization

      Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
    • Target information above from: UniProt accession Q7KZI7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2011 Human
      • Entrez Gene: 13728 Mouse
      • Entrez Gene: 60328 Rat
      • Omim: 600526 Human
      • SwissProt: Q7KZI7 Human
      • SwissProt: Q05512 Mouse
      • SwissProt: O08679 Rat
      • Unigene: 567261 Human
      • Unigene: 258986 Mouse
      • Unigene: 42926 Rat
      see all
    • Alternative names

      • ELKL motif kinase 1 antibody
      • ELKL motif kinase antibody
      • EMK-1 antibody
      • EMK1 antibody
      • MAP/microtubule affinity regulating kinase 2 antibody
      • MAP/microtubule affinity-regulating kinase 2 antibody
      • Mark2 antibody
      • MARK2_HUMAN antibody
      • MGC99619 antibody
      • PAR 1 antibody
      • Par 1b antibody
      • PAR1 homolog antibody
      • Par1b antibody
      • Ser/Thr protein kinase PAR 1B antibody
      • Serine/threonine protein kinase EMK antibody
      • Serine/threonine protein kinase MARK2 antibody
      • Serine/threonine-protein kinase MARK2 antibody
      see all

    Images

    • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
      Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: Raw264.7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
      Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
      All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

      Lane 1 : RAW 264.7 cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : Raji cell lysate
      Lane 6 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 88 kDa

    • Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)
      Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)
      Flow cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
    • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
      OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-MARK2 antibody [EPR8553] (ab133724)
      Anti-MARK2 antibody [EPR8553] (ab133724)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab133724? Please let us know so that we can cite the reference in this datasheet.

    ab133724 has been referenced in 3 publications.

    • Xu L  et al. The inhibition of MARK2 suppresses cisplatin resistance of osteosarcoma stem cells by regulating DNA damage and repair. J Bone Oncol 23:100290 (2020). PubMed: 32368441
    • Wei X  et al. MARK2 enhances cisplatin resistance via PI3K/AKT/NF-?B signaling pathway in osteosarcoma cells. Am J Transl Res 12:1807-1823 (2020). PubMed: 32509178
    • Ooki T  et al. High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b. Dev Cell 49:590-604.e9 (2019). PubMed: 31080060

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-MARK2 antibody [EPR8553]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Sample
    Mouse Cell lysate - whole cell (EpH4 mammary epithelial cells)
    Specification
    EpH4 mammary epithelial cells
    Treatment
    48hrs post siRNA transfection
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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