Overview

  • Product name

    Anti-MARK2 antibody [EPR8553]
    See all MARK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8553] to MARK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human MARK2 (Q7KZI7).

  • Positive control

    • Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
    • Tissue specificity

      High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Cellular localization

      Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ELKL motif kinase 1 antibody
      • ELKL motif kinase antibody
      • EMK-1 antibody
      • EMK1 antibody
      • MAP/microtubule affinity regulating kinase 2 antibody
      • MAP/microtubule affinity-regulating kinase 2 antibody
      • Mark2 antibody
      • MARK2_HUMAN antibody
      • MGC99619 antibody
      • PAR 1 antibody
      • Par 1b antibody
      • PAR1 homolog antibody
      • Par1b antibody
      • Ser/Thr protein kinase PAR 1B antibody
      • Serine/threonine protein kinase EMK antibody
      • Serine/threonine protein kinase MARK2 antibody
      • Serine/threonine-protein kinase MARK2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: Raw264.7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

      Lane 1 : RAW 264.7 cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : Raji cell lysate
      Lane 6 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 88 kDa

    • Flow cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133724 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Sample
    Mouse Cell lysate - whole cell (EpH4 mammary epithelial cells)
    Specification
    EpH4 mammary epithelial cells
    Treatment
    48hrs post siRNA transfection
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Jun 22 2014

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