Recombinant Anti-MARK2 antibody [EPR8553] (ab133724)
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Overview
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Product name
Anti-MARK2 antibody [EPR8553]
See all MARK2 primary antibodies -
Description
Rabbit monoclonal [EPR8553] to MARK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of Human MARK2 (Q7KZI7).
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Positive control
- Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.70 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8553 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 88 kDa. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
Tissue specificity
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Cellular localization
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
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Database links
- Entrez Gene: 2011 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 60328 Rat
- Omim: 600526 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q05512 Mouse
- SwissProt: O08679 Rat
- Unigene: 567261 Human
see all -
Alternative names
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
see all
Images
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![Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-266791-anti-mark2-antibody-epr8553-western-blot.jpg)
Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Raw264.7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147771-anti-mark2-antibody-epr8553-western-blot.jpg)
Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution
Lane 1 : RAW 264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Raji cell lysate
Lane 6 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa -
![Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147770-anti-mark2-antibody-epr8553-flow-cytometry.jpg)
Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)Flow cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green). -
![Other - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147769-anti-mark2-antibody-epr8553.jpg)
Other - Anti-MARK2 antibody [EPR8553] (ab133724)
Datasheets and documents
References
ab133724 has not yet been referenced specifically in any publications.
