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    mark2-antibody-epr8553-ab133724.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MARK
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MARK2 antibody [EPR8553] (ab133724)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (ab133724)
  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
  • Anti-MARK2 antibody [EPR8553] (ab133724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8553] to MARK2
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
Product image
Recombinant human MARK2 protein (ab119135)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-p400 antibody (ab70301)

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Overview

  • Product name

    Anti-MARK2 antibody [EPR8553]
    See all MARK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8553] to MARK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.70 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8553
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MARK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Alternative Versions

    • Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human MARK2 protein (ab119135)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Application notes
Is unsuitable for ICC,IHC-P or IP.

Target

  • Function

    Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
  • Tissue specificity

    High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localization

    Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
  • Target information above from: UniProt accession Q7KZI7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2011 Human
    • Entrez Gene: 13728 Mouse
    • Entrez Gene: 60328 Rat
    • Omim: 600526 Human
    • SwissProt: Q7KZI7 Human
    • SwissProt: Q05512 Mouse
    • SwissProt: O08679 Rat
    • Unigene: 567261 Human
    • Unigene: 258986 Mouse
    • Unigene: 42926 Rat
    see all
  • Alternative names

    • ELKL motif kinase 1 antibody
    • ELKL motif kinase antibody
    • EMK-1 antibody
    • EMK1 antibody
    • MAP/microtubule affinity regulating kinase 2 antibody
    • MAP/microtubule affinity-regulating kinase 2 antibody
    • Mark2 antibody
    • MARK2_HUMAN antibody
    • MGC99619 antibody
    • PAR 1 antibody
    • Par 1b antibody
    • PAR1 homolog antibody
    • Par1b antibody
    • Ser/Thr protein kinase PAR 1B antibody
    • Serine/threonine protein kinase EMK antibody
    • Serine/threonine protein kinase MARK2 antibody
    • Serine/threonine-protein kinase MARK2 antibody
    see all

Images

  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Raw264.7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

  • Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (ab133724)
    Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (ab133724)

    Intracellular Flow Cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).

     

  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
    OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MARK2 antibody [EPR8553] (ab133724)
    Anti-MARK2 antibody [EPR8553] (ab133724)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab133724? Please let us know so that we can cite the reference in this datasheet.

ab133724 has been referenced in 4 publications.

  • Ooki T & Hatakeyama M Protocol for visualizing conditional interaction between transmembrane and cytoplasmic proteins. STAR Protoc 2:100430 (2021). PubMed: 33870231
  • Xu L  et al. The inhibition of MARK2 suppresses cisplatin resistance of osteosarcoma stem cells by regulating DNA damage and repair. J Bone Oncol 23:100290 (2020). PubMed: 32368441
  • Wei X  et al. MARK2 enhances cisplatin resistance via PI3K/AKT/NF-?B signaling pathway in osteosarcoma cells. Am J Transl Res 12:1807-1823 (2020). PubMed: 32509178
  • Ooki T  et al. High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b. Dev Cell 49:590-604.e9 (2019). PubMed: 31080060

Customer reviews and Q&As

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Western blot abreview for Anti-MARK2 antibody [EPR8553]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Mouse Cell lysate - whole cell (EpH4 mammary epithelial cells)
Specification
EpH4 mammary epithelial cells
Treatment
48hrs post siRNA transfection
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jun 22 2014

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