Recombinant Anti-MARK2 antibody [EPR8553] (ab133724)
![Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-266791-anti-mark2-antibody-epr8553-western-blot.jpg "Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8553] to MARK2
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MARK2 antibody [EPR8553]
See all MARK2 primary antibodies -
Description
Rabbit monoclonal [EPR8553] to MARK2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.70 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8553 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa. |
Target
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Function
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
Tissue specificity
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Cellular localization
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
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Database links
- Entrez Gene: 2011 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 60328 Rat
- Omim: 600526 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q05512 Mouse
- SwissProt: O08679 Rat
- Unigene: 567261 Human
see all -
Alternative names
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
see all
Images
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Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Raw264.7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147771-anti-mark2-antibody-epr8553-western-blot.jpg)
Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution
Lane 1 : RAW 264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Raji cell lysate
Lane 6 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa -
![Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147770-anti-mark2-antibody-epr8553-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (ab133724)Intracellular Flow Cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
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![OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-147769-anti-mark2-antibody-epr8553.jpg)
OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724) -
![Anti-MARK2 antibody [EPR8553] (ab133724)](https://www.abcam.com/ps/products/133/ab133724/Images/ab133724-5-benefits-of-recombinant-antibodies.png)
Anti-MARK2 antibody [EPR8553] (ab133724)
Datasheets and documents
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Datasheet download
References (4)
ab133724 has been referenced in 4 publications.
- Ooki T & Hatakeyama M Protocol for visualizing conditional interaction between transmembrane and cytoplasmic proteins. STAR Protoc 2:100430 (2021). PubMed: 33870231
- Xu L et al. The inhibition of MARK2 suppresses cisplatin resistance of osteosarcoma stem cells by regulating DNA damage and repair. J Bone Oncol 23:100290 (2020). PubMed: 32368441
- Wei X et al. MARK2 enhances cisplatin resistance via PI3K/AKT/NF-?B signaling pathway in osteosarcoma cells. Am J Transl Res 12:1807-1823 (2020). PubMed: 32509178
- Ooki T et al. High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b. Dev Cell 49:590-604.e9 (2019). PubMed: 31080060
