Key features and details
- Rabbit polyclonal to MARK2 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Product nameAnti-MARK2 antibody - N-terminal
See all MARK2 primary antibodies
DescriptionRabbit polyclonal to MARK2 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to amino acids within the N-terminal of Human MARK2.
- Human kidney tubule tissue; A549 cells; COS7 cell extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab136872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 88 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionRole in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
Tissue specificityHigh levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Cellular localizationCell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
- Information by UniProt
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
All lanes : Anti-MARK2 antibody - N-terminal (ab136872) at 1/500 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with synthesised peptide
Predicted band size: 88 kDa
Immunohistochemical analysis of paraffin embedded Human kidney tubules labelling ab136872 at 10µg/ml.
Immunofluorescence of A549 cells labelling ab136872 at 1/100 dilution. Image on the right is treated with synthesised peptide
ab136872 has not yet been referenced specifically in any publications.