Anti-MARK2 antibody - N-terminal (ab136872)
Key features and details
- Rabbit polyclonal to MARK2 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-MARK2 antibody - N-terminal
See all MARK2 primary antibodies -
Description
Rabbit polyclonal to MARK2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to amino acids within the N-terminal of Human MARK2.
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Positive control
- Human kidney tubule tissue; A549 cells; COS7 cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 88 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 88 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
Tissue specificity
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Cellular localization
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
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Database links
- Entrez Gene: 2011 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 60328 Rat
- Omim: 600526 Human
- SwissProt: Q7KZI7 Human
- SwissProt: Q05512 Mouse
- SwissProt: O08679 Rat
- Unigene: 567261 Human
see all -
Alternative names
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
see all
Images
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All lanes : Anti-MARK2 antibody - N-terminal (ab136872) at 1/500 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with synthesised peptide
Predicted band size: 88 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tubules labelling ab136872 at 10µg/ml.
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Immunofluorescence of A549 cells labelling ab136872 at 1/100 dilution. Image on the right is treated with synthesised peptide
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136872 has not yet been referenced specifically in any publications.