Anti-MARK4 antibody (ab5262)

Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. We realise that these products might be very suitable for your research and do not wish to delay this; therefore, we release them early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. Preliminary Western blot testing with ab5262 showed the following: no signal obtained yet but low background observed in Human Brain and 293 lysates at up to 2µg/ml. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abcam points to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any additional questions, just let us know.

Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. Important parts of the protocol: - Plates coated with immunizing peptide at 1ug/ml, 4C overnight - Plates blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature - Secondary antibody, Anti-goat Alkaline Phoshatase - Readout using p-nitrophenyl phosphate in diethanolamine buffer. Re MARK 1, 2, 3 currently we aren't making them currently. However, there are at least 10 antibodies against Tau (ab4841, ab4749, ab4846, ab4856, ab4858). We hope this will help!