• Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab5262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
    • Tissue specificity
      Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Developmental stage
      Expressed at all stages of the mitotic cell cycle.
    • Post-translational
      Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
      Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ90097 antibody
      • KIAA1860 antibody
      • MAP / microtubule affinity regulating kinase 4 antibody
      • MAP / microtubule affinity regulating kinase 4L antibody
      • MAP/microtubule affinity regulating kinase 4 antibody
      • MAP/microtubule affinity regulating kinase like 1 antibody
      • MAP/microtubule affinity-regulating kinase 4 antibody
      • MAP/microtubule affinity-regulating kinase-like 1 antibody
      • MARK 4 antibody
      • MARK 4L antibody
      • MARK L1 antibody
      • MARK4 antibody
      • MARK4 serine / threonine protein kinase antibody
      • MARK4 serine/threonine protein kinase antibody
      • MARK4_HUMAN antibody
      • MARK4S antibody
      • MARKL 1 antibody
      • MARKL1 antibody
      • Nbla00650 antibody
      • Putative protein product of Nbla00650 antibody
      see all


    • Anti-MARK4 antibody (ab5262) at 0.5 µg + Human Prostate lysate (35µg protein in RIPA buffer).

      Predicted band size: 75 kDa
      Observed band size: 70 kDa (why is the actual band size different from the predicted?)

      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-...

    Read More

    Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. I...

    Read More


    Sign up