Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
    • Tissue specificity
      Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Developmental stage
      Expressed at all stages of the mitotic cell cycle.
    • Post-translational
      modifications
      Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
      Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ90097 antibody
      • KIAA1860 antibody
      • MAP / microtubule affinity regulating kinase 4 antibody
      • MAP / microtubule affinity regulating kinase 4L antibody
      • MAP/microtubule affinity regulating kinase 4 antibody
      • MAP/microtubule affinity regulating kinase like 1 antibody
      • MAP/microtubule affinity-regulating kinase 4 antibody
      • MAP/microtubule affinity-regulating kinase-like 1 antibody
      • MARK 4 antibody
      • MARK 4L antibody
      • MARK L1 antibody
      • MARK4 antibody
      • MARK4 serine / threonine protein kinase antibody
      • MARK4 serine/threonine protein kinase antibody
      • MARK4_HUMAN antibody
      • MARK4S antibody
      • MARKL 1 antibody
      • MARKL1 antibody
      • Nbla00650 antibody
      • Putative protein product of Nbla00650 antibody
      see all

    Images

    • Anti-MARK4 antibody (ab5262) at 0.5 µg + Human Prostate lysate (35µg protein in RIPA buffer).

      Predicted band size: 75 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Wang L  et al. Prazosin protects myocardial cells against anoxia-reoxygenation injury via the extracellular signal-regulated kinase signaling pathway. Mol Med Rep 17:2145-2152 (2018). Read more (PubMed: 29207167) »
    • Magnani I  et al. Differential signature of the centrosomal MARK4 isoforms in glioma. Anal Cell Pathol (Amst) 34:319-38 (2011). Read more (PubMed: 22156016) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. We realise that these products might be very suitable for your research and do not wish to delay this; therefore, we release them early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. Preliminary Western blot testing with ab5262 showed the following: no signal obtained yet but low background observed in Human Brain and 293 lysates at up to 2µg/ml. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abcam points to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any additional questions, just let us know.

    Read More

    Answer

    Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. Important parts of the protocol: - Plates coated with immunizing peptide at 1ug/ml, 4C overnight - Plates blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature - Secondary antibody, Anti-goat Alkaline Phoshatase - Readout using p-nitrophenyl phosphate in diethanolamine buffer. Re MARK 1, 2, 3 currently we aren't making them currently. However, there are at least 10 antibodies against Tau (ab4841, ab4749, ab4846, ab4856, ab4858). We hope this will help!

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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