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    mark4-antibody-ab5262.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MARK
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Anti-MARK4 antibody (ab5262)

  • Datasheet
Submit a review Q&A (2)References (3)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-MARK4 antibody (ab5262)

    Key features and details

    • Goat polyclonal to MARK4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human MARK4 protein (ab105211)
    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-MARK4 antibody
      See all MARK4 primary antibodies
    • Description

      Goat polyclonal to MARK4
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      IKRKPPPGCSDSPGV

      , corresponding to C terminal amino acids 674-688 of Human MARK4.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.3
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • MT Associated Proteins
      • MARK

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant human MARK4 protein (ab105211)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5262 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
    • Tissue specificity

      Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Developmental stage

      Expressed at all stages of the mitotic cell cycle.
    • Post-translational
      modifications

      Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
      Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
    • Cellular localization

      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
    • Target information above from: UniProt accession Q96L34 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57787 Human
      • Omim: 606495 Human
      • SwissProt: Q96L34 Human
      • Unigene: 34314 Human
      • Alternative names

        • FLJ90097 antibody
        • KIAA1860 antibody
        • MAP / microtubule affinity regulating kinase 4 antibody
        • MAP / microtubule affinity regulating kinase 4L antibody
        • MAP/microtubule affinity regulating kinase 4 antibody
        • MAP/microtubule affinity regulating kinase like 1 antibody
        • MAP/microtubule affinity-regulating kinase 4 antibody
        • MAP/microtubule affinity-regulating kinase-like 1 antibody
        • MARK 4 antibody
        • MARK 4L antibody
        • MARK L1 antibody
        • MARK4 antibody
        • MARK4 serine / threonine protein kinase antibody
        • MARK4 serine/threonine protein kinase antibody
        • MARK4_HUMAN antibody
        • MARK4S antibody
        • MARKL 1 antibody
        • MARKL1 antibody
        • Nbla00650 antibody
        • Putative protein product of Nbla00650 antibody
        see all

      Images

      • Western blot - Anti-MARK4 antibody (ab5262)
        Western blot - Anti-MARK4 antibody (ab5262)
        Anti-MARK4 antibody (ab5262) at 0.5 µg + Human Prostate lysate (35µg protein in RIPA buffer).

        Predicted band size: 75 kDa
        Observed band size: 70 kDa
        why is the actual band size different from the predicted?



        Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (3)

      Publishing research using ab5262? Please let us know so that we can cite the reference in this datasheet.

      ab5262 has been referenced in 3 publications.

      • Chen Y  et al. Capn4 regulates migration and invasion of ovarian carcinoma cells via targeting osteopontin-mediated PI3K/AKT signaling pathway. Oncol Lett 17:564-570 (2019). PubMed: 30655802
      • Wang L  et al. Prazosin protects myocardial cells against anoxia-reoxygenation injury via the extracellular signal-regulated kinase signaling pathway. Mol Med Rep 17:2145-2152 (2018). PubMed: 29207167
      • Magnani I  et al. Differential signature of the centrosomal MARK4 isoforms in glioma. Anal Cell Pathol (Amst) 34:319-38 (2011). PubMed: 22156016

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I have a few questions regarding the product: What region was it made against? Which epitope was it made against? In which cell lines(s) does the protein express that could be used as a positive control? Does the antibody recognize a single band versus multiple bands? Thank you

      Read More

      Abcam community

      Verified customer

      Asked on May 25 2005

      Answer

      Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. We realise that these products might be very suitable for your research and do not wish to delay this; therefore, we release them early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. Preliminary Western blot testing with ab5262 showed the following: no signal obtained yet but low background observed in Human Brain and 293 lysates at up to 2µg/ml. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abcam points to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any additional questions, just let us know.

      Read More

      Abcam Scientific Support

      Answered on May 25 2005

      Question

      I am interested in your MARK4 antibody. The datasheet states it has been tested in "peptide ELISA." I would like more specific information about the ELISA format (primary, secondary antibodies, procedure, etc.). Also, I am interested in antibodies for MARK 1,2,and 3 and for tau. If you have any of these planned for fast-track availability I am very interested in testing them. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 19 2005

      Answer

      Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. Important parts of the protocol: - Plates coated with immunizing peptide at 1ug/ml, 4C overnight - Plates blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature - Secondary antibody, Anti-goat Alkaline Phoshatase - Readout using p-nitrophenyl phosphate in diethanolamine buffer. Re MARK 1, 2, 3 currently we aren't making them currently. However, there are at least 10 antibodies against Tau (ab4841, ab4749, ab4846, ab4856, ab4858). We hope this will help!

      Read More

      Abcam Scientific Support

      Answered on Jan 24 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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