Recombinant Anti-MetRS/MARS antibody [EPR9872] (ab180497)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9872] to MetRS/MARS
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MetRS/MARS antibody [EPR9872]
See all MetRS/MARS primary antibodies -
Description
Rabbit monoclonal [EPR9872] to MetRS/MARS -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human MetRS/MARS aa 150-250. The exact sequence is proprietary.
Database link: P56192 -
Positive control
- WB: HeLa, HepG2, SW480, Caco2 and PC-12 lysates. IHC-P: human thyroid carcinoma and brain tissues, and rat kidney tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9872 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/50 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
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IP |
1/20 - 1/40.
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ICC/IF |
1/50 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/20. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 |
WB
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
IP
1/20 - 1/40. |
ICC/IF
1/50 - 1/250. |
Target
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Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Contains 1 WHEP-TRS domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4141 Human
- Entrez Gene: 299851 Rat
- Omim: 156560 Human
- SwissProt: P56192 Human
- Unigene: 632707 Human
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Alternative names
- cytoplasmic antibody
- FLJ35667 antibody
- Mars antibody
see all
Images
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All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution (Purified)
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDa -
All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/50000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDa -
All lanes : Anti-MetRS/MARS antibody [EPR9872] (ab180497) at 1/10000 dilution ((unpurified))
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : Caco2 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/50 dilution (4.52 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab180497 (purified ) at 1/20 dilution (1ug) immunoprecipitating MetRS/MARS in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab180497 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180497 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MetRS/MARS with purified ab180497 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling MetRS/MARS with purified ab180497 at 1/4000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunofluorescent analysis of HeLa cells (acetone-fixed) labeling MARS with unpurified ab180497 at 1/250 dilution (green) followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution and DAPI staining (blue).
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MARS with unpurified ab180497 at 1/50 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180497 at 1/40 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab180497 has been referenced in 2 publications.
- Hei Z et al. Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. J Biol Chem 294:4775-4783 (2019). PubMed: 30733335
- Abuduxikuer K et al. Novel methionyl-tRNA synthetase gene variants/phenotypes in interstitial lung and liver disease: A case report and review of literature. World J Gastroenterol 24:4208-4216 (2018). PubMed: 30271085