Product nameAnti-MARS2 antibody
DescriptionRabbit polyclonal to MARS2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- RT4 cell lysates; Human stomach tissue.
General notesab130426 is a mono-specific antibody.
The antibody solution should be gently mixed before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab130426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500. Predicted molecular weight: 67 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- mars2 antibody
- Methionine tRNA ligase 2 antibody
- Methionine tRNA ligase 2 mitochondrial antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) and aggresome. Recommended concentration of ab130426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-MARS2 antibody (ab130426) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 67 kDa
ab130426, at a dilution of 1/200, staining MARS2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab130426 has not yet been referenced specifically in any publications.