Key features and details
- Rabbit polyclonal to MARVELD2/Tricellulin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MARVELD2/Tricellulin antibody
See all MARVELD2/Tricellulin primary antibodies
DescriptionRabbit polyclonal to MARVELD2/Tricellulin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese hamster, Common marmoset, Orangutan
- HeLa whole cell lysate.
This product was previously labelled as MARVELD2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176812 was affinity purified using an epitope specific to MARVELD2/Tricellulin immobilized on solid support.
Our Abpromise guarantee covers the use of ab176812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceTight junctions (TJ) prevent leakage of solutes through the paracellular pathway of epithelial cells. MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts. It plays a role in the formation of the epithelial barriers.
Cellular localizationCell membrane; Multi-pass membrane protein. Cell junction, tight junction. Note=Found at tricellular contacts.
- DFNB49 antibody
- FLJ30532 antibody
- MARVD2 antibody
Detection of MARVELD2/Tricellulin in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176812 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176812 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-MARVELD2/Tricellulin antibody (ab176812) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
ab176812 has not yet been referenced specifically in any publications.