Recombinant Anti-MAS1L antibody [EPR11440(B)] (ab156018)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11440(B)] to MAS1L
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MAS1L antibody [EPR11440(B)]
See all MAS1L primary antibodies -
Description
Rabbit monoclonal [EPR11440(B)] to MAS1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human MAS1L (UniProt P35410).
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Positive control
- HepG2, 293T, SH-SY5Y, Y79 and HeLa cell lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11440(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
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IP |
1/100 - 1/500.
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Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 42 kDa. |
IP
1/100 - 1/500. |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Mas subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 116511 Human
- Omim: 607235 Human
- SwissProt: P35410 Human
- Unigene: 533297 Human
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Alternative names
- dJ994E9.2 antibody
- MAS L antibody
- MAS R antibody
see all
Images
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All lanes : Anti-MAS1L antibody [EPR11440(B)] (ab156018) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Y79 cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
Flow cytometric analysis of permeabilized 293T cells labeling MAS1L with ab156018 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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Immunoprecipitation analysis of HepG2 cell lysates using ab156018 at 1/100 dilution (Lane 1) or rabbit IgG (Lane 2).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab156018 has been referenced in 1 publication.
- Huang H et al. Angiotensin II type 1 receptor-associated protein plays a role in regulating the local renin-angiotensin system in HSC-T6 cells. Mol Med Rep 12:3763-3768 (2015). PubMed: 26018598