Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886)
Key features and details
- Rabbit polyclonal to MASH1/Achaete-scute homolog 1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-MASH1/Achaete-scute homolog 1 antibody
See all MASH1/Achaete-scute homolog 1 primary antibodies -
Description
Rabbit polyclonal to MASH1/Achaete-scute homolog 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human MASH1/Achaete-scute homolog 1 (internal sequence). The exact sequence is proprietary.
Database link: P50553 -
Positive control
- WB: SK N SH whole cell lysate (ab3956), SH-SY5Y, PC-12 and Rat2 whole cell extracts; SH-SY5Y nuclear extract. IHC-P: Rat brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
IHC-P
1/100 - 1/1000. |
Target
-
Function
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3'). -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 429 Human
- Entrez Gene: 64186 Rat
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: P19359 Rat
- Unigene: 703025 Human
- Unigene: 32936 Rat
-
Alternative names
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
Images
-
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
Paraffin-embedded rat brain tissue stained for MASH1/Achaete-scute homolog 1 using ab227886 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
-
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa15 % SDS-PAGE gel.
-
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab227886 has not yet been referenced specifically in any publications.