Overview

  • Product name

    Anti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description

    Rabbit polyclonal to MASH1/Achaete-scute homolog 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human MASH1/Achaete-scute homolog 1 (internal sequence). The exact sequence is proprietary.
    Database link: P50553

  • Positive control

    • WB: SK N SH whole cell lysate (ab3956), SH-SY5Y, PC-12 and Rat2 whole cell extracts; SH-SY5Y nuclear extract. IHC-P: Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute family bHLH transcription factor 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Images

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
    Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded rat brain tissue stained for MASH1/Achaete-scute homolog 1 using ab227886 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa



    15 % SDS-PAGE gel.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

References

ab227886 has not yet been referenced specifically in any publications.

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