Key features and details
- Goat polyclonal to MASH1/Achaete-scute homolog 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MASH1/Achaete-scute homolog 1 antibody
See all MASH1/Achaete-scute homolog 1 primary antibodies
DescriptionGoat polyclonal to MASH1/Achaete-scute homolog 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: MASH1/Achaete-scute homolog 1 human recombinant protein C-MYC/DDK-tagged; SH-SY5Y whole cell lysate. IHC-P: Human thyroid papillary carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 25 kDa.|
FunctionMay play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
Human thyroid papillary carcinoma tissue stained for MASH1/Achaete-scute homolog 1 with ab235198 at a 1/1000 dilution in immunohistochemical analysis. Counterstained with hematoxylin. Antigen retrieval HIER pH 6. No fixative.
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution
Lane 1 : Mouse pancreas lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Anti-Goat IgG (H&L) (Rabbit) peroxidase conjugated at 1/5000 dilution
Predicted band size: 25 kDa
Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution + MASH1/Achaete-scute homolog 1 human recombinant protein C-MYC/DDK-tagged, 50 ng
Donkey Fab2-anti-Goat [H&L] mx8 HRP at 1/5000 dilution
Predicted band size: 25 kDa
Exposure time: 15 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235198 has not yet been referenced specifically in any publications.