Overview

  • Product name

    Anti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description

    Goat polyclonal to MASH1/Achaete-scute homolog 1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: MASH1/Achaete-scute homolog 1 human recombinant protein C-MYC/DDK-tagged; SH-SY5Y whole cell lysate. IHC-P: Human thyroid papillary carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 25 kDa.

Target

  • Function

    May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute family bHLH transcription factor 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Images

  • Human thyroid papillary carcinoma tissue stained for MASH1/Achaete-scute homolog 1  with ab235198 at a 1/1000 dilution in immunohistochemical analysis. Counterstained with hematoxylin. Antigen retrieval  HIER pH 6. No fixative.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution

    Lane 1 : Mouse pancreas lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Anti-Goat IgG (H&L) (Rabbit) peroxidase conjugated at 1/5000 dilution

    Predicted band size: 25 kDa

  • Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution + MASH1/Achaete-scute homolog 1 human recombinant protein C-MYC/DDK-tagged, 50 ng

    Secondary
    Donkey Fab2-anti-Goat [H&L] mx8 HRP at 1/5000 dilution

    Predicted band size: 25 kDa


    Exposure time: 15 seconds

References

ab235198 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab235198.
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