Overview

  • Product name
    Anti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to MASH1/Achaete-scute homolog 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MASH1/Achaete-scute homolog 1 aa 166-196 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P50553

  • Positive control
    • WB: Mouse lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24947819
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute family bHLH transcription factor 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Images

  • Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556) at 1/500 dilution + Mouse lung tissue lysate

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 15 kDa, 28 kDa, 45 kDa (possible dimer), 55 kDa (possible dimer). We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of mouse adult NSCs derived from SVZ, staining TTF1 with ab38556.

    Cells were fixed with Zamboni's fixative, permeabilized with 0.5% Triton X-100 and blocked with 3% goat serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/500 in 3% goat serum) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Miyamoto Y  et al. VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. Nat Commun 7:13478 (2016). Read more (PubMed: 27876794) »
  • Sandstrom RS  et al. Epigenetic Regulation by Chromatin Activation Mark H3K4me3 in Primate Progenitor Cells within Adult Neurogenic Niche. Sci Rep 4:5371 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24947819) »
See all 5 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Please thank your customer for getting back to us. I am very happy to hear that the staining with Anti-MASH1/Achaete-scute homolog 1 antibody gives a good result.

The image shows the nuclear localization of the protein very nicely.

Maybe your customer would like to write an Abreview about their experiments and earn Abpoints that they can redeem.

Please let me know if I can be of any more help.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (adult NSC derived from SVZ (neurosphere))
Specification
adult NSC derived from SVZ (neurosphere)
Fixative
Zamboni's Fixative
Permeabilization
Yes - 0.5% TritonX-100 for 10min on ice
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Michael Weible

Verified customer

Submitted Nov 08 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 03 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 03 2012

Answer

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results from these antibodies.

1.) Both antibodies were used after PFA fixation and paraffin wax embedding anda 10 mM sodium citrate, pH 6.0 antigen retrieval.

2.) I suggest to lower the concentration of tritonX to 0.1 to 0.2% for 10 minutes since it is a very harsh detergent and might damage the target protein.

3.) I can also suggest to use a standard tissue as positive control in case the neurospheres are difficult to handle.

4.) I attached the protocol these two antibodies were used with to this email for your information. It is a fairly standard protocol and might therefore need optimization when extraordinary samples like neurospheres are used.

We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Answer

Gracias por tu respuesta.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo los anticuerpos ab108937 y ab135273. Recibiréis una confirmación automática del mismo con más detalles.

Si necesitáis cualquier información referente al pedido, no dudéis en contactarnos, referenciando el número de éste: XXXXX.

Respecto al protocolo a seguir, no hay ninguno concreto para realizar inmunos con estos anticuerpos. Puedes seguir las pautas mencionadas en correos anteriores, y si te sirve de ayuda, puedes también referirte a los https://www.abcam.com/index.html?pageconfig=resource&rid=11384 que tenemos en nuestra Web.

Si tuvierais cualquier otro problema, o requirierais mas información / asistencia, por favor, no dudéis en volvernos a contactar.

Read More

Answer

Muchas gracias por tu respuesta.

He comentado el protocolo seguido en IHC-P con mis compañeros del laboratorio, para tratar de entender el problema ocurrido con Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556). Sin embargo me confirman que parece ser todo correcto desde su punto de vista, y lamentablemente no tenemos ninguna otra sugerencia posible.

Si os parece en el mismo envío que os haga del anticuerpo ab119776 (Anti-Hes1) puedo además mandaros otro anti MASH1 testado en IHC-P, como última alternativa para poder visualizar la proteína en vuestros tejidos.

https://www.abcam.com/MASH1Achaete-scute-homolog-1-antibody-ab135273.html

Confírmamelo y realizo el pedido de inmediato. Espero tu respuesta.

Read More

Answer

Gracias por contactarnos de nuevo.

Lamento mucho que sigas teniendo problemas con dos de los anticuerpos enviados, y te agradezco que nos hayas enviado las imágenes.

En relación al MASH1, me temo que no puedo ofrecerte más recomendaciones a la hora de usarlo, puesto que en el laboratorio no se ha validado en secciones de parafina. Es posible de hecho que el anticuerpo no reconozca la proteína en estas condiciones. Al ser un policlonal, hay bastante variación de unos lotes a otros, y puede que a pesar de que en el pasado a otro usuario le funcionara en IHC-P, este nuevo lote no lo haga. En cualquier caso, no puedo asegurártelo puesto que no se testa en el laboratorio en esta aplicación.

Puedes, si tienes oportunidad, teñir un control positivo (carcinoma de mama o tejido de pulmón de ratón) o usarlo en otra aplicación validada (ICC, WB o IHC-FoFr) para comprobar su funcionamiento.

Respecto al anticuerpo contra Hes1 podemos echar un vistazo al protocolo seguido e intentar darte alguna sugerencia que pueda optimizar el resultado.

Muchas gracias por tu colaboración. Espero tu respuesta.

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Answer



Expiration date: 2012-11-09


I am very pleased to hear you would like to accept our offer and test

ab38556 Anti-MASH1/Achaete-scute homolog 1 antibody
ab39717 Anti-Plexin B1 antibody
ab117546 Anti-Dlx2 antibody [4B9]



in ICC. One code will give you: 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for ICCand include this code in the "Additional Comments" section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.


In addition, ab97959 Anti-SOX2 antibodyhas already been tested for mouse as well as for ICC. Therefore, itis not a testing-discount candidate.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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